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The Genetic Transformation of Sweet Orange (Citrus sinensis L. Osbeck) for Enhanced Resistance to Citrus Canker

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Transgenic Plants

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1864))

Abstract

Developing disease resistance is one of the most important components of any plant breeding program. Citrus traditional breeding methods (bud sport selection, crossbreeding, and other breeding channels) are a laborious task and often hampered by long juvenility, a high degree of heterozygosity, polyembryony, self-incompatibility, and abortion of reproductive organs. An interesting alternative to the classical breeding approach is the use of genetic transformation, which provides the means for adding a single agronomic trait to a plant without otherwise altering its phenotype. Agrobacterium tumefaciens-mediated transformation has been carried out with numerous hybrids and citrus species. This technique allowed us to introduce the Bs2 gene in Citrus, as well as to increase citrus canker resistance in transgenic Bs2 gene-expressing lines.

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Acknowledgments

This project was supported by Estación Experimental Agroindustrial Obispo Colombres (EEAOC) and Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET).

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Authors and Affiliations

  1. Instituto de Tecnología Agroindustrial del Noroeste Argentino (ITANOA), EEAOC-CONICET, Av. William Cross 3150, Las Talitas, T4101XAC, Tucumán, Argentina

    Lorena Noelia Sendin & María Paula Filippone

Authors
  1. Lorena Noelia Sendin
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  2. María Paula Filippone
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Corresponding author

Correspondence to María Paula Filippone .

Editor information

Editors and Affiliations

  1. Corteva Agriscience™, Agriculture Division of DowDuPont™, Johnston, IA, USA

    Sandeep Kumar

  2. Corteva Agriscience™, Agriculture Division of DowDuPont™, Johnston, IA, USA

    Pierluigi Barone

  3. Corteva Agriscience™, Agriculture Division of DowDuPont™, Johnston, IA, USA

    Michelle Smith

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Sendin, L.N., Filippone, M.P. (2019). The Genetic Transformation of Sweet Orange (Citrus sinensis L. Osbeck) for Enhanced Resistance to Citrus Canker. In: Kumar, S., Barone, P., Smith, M. (eds) Transgenic Plants. Methods in Molecular Biology, vol 1864. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8778-8_13

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  • DOI: https://doi.org/10.1007/978-1-4939-8778-8_13

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8777-1

  • Online ISBN: 978-1-4939-8778-8

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