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Measuring Antibody-Antigen Binding Kinetics Using Surface Plasmon Resonance

  • Stephen Hearty
  • Paul Leonard
  • Hui Ma
  • Richard O’KennedyEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1827)

Abstract

Surface plasmon resonance (SPR) is now widely embraced as a technology for monitoring a diverse range of protein-protein interactions and is considered almost de rigueur for characterizing antibody-antigen interactions. The technique obviates the need to label either of the interacting species, and the binding event is visualized in real time. Thus, it is ideally suited for screening crude, unpurified antibody samples that dominate early candidate panels following antibody selection campaigns. SPR returns not only concentration and affinity data but when used correctly can resolve the discrete component kinetic parameters (association and dissociation rate constants) of the affinity interaction. Herein, we outline some SPR-based generic antibody screening configurations and methodologies in the context of expediting data-rich ranking of candidate antibody panels and ensuring that antibodies with the optimal kinetic binding characteristics are reliably identified.

Key words

Surface plasmon resonance (SPR) Antibody Affinity Antigen Association rate Dissociation rate Screening 

Notes

Acknowledgments

The authors gratefully acknowledge the technical contributions from C. J. Hayes, P. J. Conroy, and B. Vijayalakshmi Ayyar. This material is based on work supported by the Science Foundation Ireland (grants 10/CE/B1821 and 14/IA/2646) and Enterprise Ireland (grant CF/2015/0105).

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Stephen Hearty
    • 1
    • 2
  • Paul Leonard
    • 1
    • 2
  • Hui Ma
    • 1
  • Richard O’Kennedy
    • 1
    • 2
    • 3
    Email author
  1. 1.School of BiotechnologyDublin City UniversityDublinIreland
  2. 2.National Centre for Sensor ResearchDublin City UniversityDublinIreland
  3. 3.Qatar Foundation and Research ComplexHamad Bin Khalifa University, Education CityDohaQatar

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