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Epitope Mapping by Surface Plasmon Resonance

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Epitope Mapping Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 524))

Summary

Biacore™ systems (Biacore AB) enable label-free detection of molecular interactions in real time using surface plasmon resonance detection. Epitope mapping of antibodies is usually performed in a pairwise fashion where one antibody is used to capture the antigen from solution and the binding of a secondary antibody is monitored. In contrast to alternative approaches, the method allows for mapping of large matrices of antibody pairs without the need for cumbersome labeling steps, and the real-time analysis enables a better ranking of complex stability when compared with end-point assays.

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Authors and Affiliations

  1. Department of Systems and Applications, Biacore AB (a GE Healthcare Company), Rapsgatan 7, 754 50, Uppsala, Sweden

    Pär Säfsten

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  1. Pär Säfsten
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Correspondence to Pär Säfsten .

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    © 2009 Humana Press, a part of Springer Science+Business Media, LLC

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    Säfsten, P. (2009). Epitope Mapping by Surface Plasmon Resonance. In: Schutkowski, M., Reineke, U. (eds) Epitope Mapping Protocols. Methods in Molecular Biology™, vol 524. Humana Press. https://doi.org/10.1007/978-1-59745-450-6_5

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    • DOI: https://doi.org/10.1007/978-1-59745-450-6_5

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    • Publisher Name: Humana Press

    • Print ISBN: 978-1-934115-17-6

    • Online ISBN: 978-1-59745-450-6

    • eBook Packages: Springer Protocols

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