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Proteomics as a Tool to Study Molecular Changes During Plant Morphogenesis In Vitro

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Plant Cell Culture Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1815))

Abstract

Proteome analysis represents a promising approach for plant tissue culture since it is now possible to identify and quantify proteins on a large scale. Biomarker discovery and the study of the molecular events associated with in vitro plant morphogenesis are considered potential targets for application of proteomics technologies. This chapter describes a protocol for application in in vitro plant material using two proteomics approaches: 2-DE coupled to mass spectrometry and liquid chromatography-linked tandem mass spectrometry.

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References

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Acknowledgments

This work was supported by grants from São Paulo Research Foundation—FAPESP (Proc. 15/21075-4), Carlos Chagas Filho Foundation for Research Support in the State of Rio de Janeiro—FAPERJ (Proc. E26/201.574/2014) and National Council for Scientific and Technological Development-CNPq (Proc. 454451/2014-8).

Author information

Authors and Affiliations

  1. Laboratory of Plant Cell Biology, Department of Botany, Institute of Biosciences, University of São Paulo, São Paulo, Brazil

    André Luis Wendt dos Santos & Eny Iochevet Segal Floh

  2. Laboratório de Biotecnologia, Centro de Biociências e Biotecnologia (CBB), Universidade Estadual do Norte Fluminense Darcy Ribeiro (UENF), Campos dos Goytacazes, RJ, Brazil

    Ricardo Souza Reis, Angelo Schuabb Heringer & Vanildo Silveira

  3. Unidade de Biologia Integrativa, Setor de Genômica e Proteômica, UENF, Campos dos Goytacazes, RJ, Brazil

    Ricardo Souza Reis, Angelo Schuabb Heringer & Vanildo Silveira

  4. Laboratório de Biologia Celular e Tecidual, CBB, UENF, Campos dos Goytacazes, RJ, Brazil

    Claudete Santa-Catarina

Authors
  1. André Luis Wendt dos Santos
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  2. Ricardo Souza Reis
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  3. Angelo Schuabb Heringer
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  4. Eny Iochevet Segal Floh
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  5. Claudete Santa-Catarina
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  6. Vanildo Silveira
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Editor information

Editors and Affiliations

  1. Centro de Investigación Científica de Yucatán, Unidad de Bioquímica y Biología, Molecular de Plantas, Mérida, Yucatán, Mexico

    Víctor M. Loyola-Vargas

  2. Departamento de Ingeniería Genética, Unidad Irapuato, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Irapuato, Guanajuato, Mexico

    Neftalí Ochoa-Alejo

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Wendt dos Santos, A.L., Souza Reis, R., Schuabb Heringer, A., Segal Floh, E.I., Santa-Catarina, C., Silveira, V. (2018). Proteomics as a Tool to Study Molecular Changes During Plant Morphogenesis In Vitro. In: Loyola-Vargas, V., Ochoa-Alejo, N. (eds) Plant Cell Culture Protocols. Methods in Molecular Biology, vol 1815. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8594-4_24

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  • DOI: https://doi.org/10.1007/978-1-4939-8594-4_24

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8593-7

  • Online ISBN: 978-1-4939-8594-4

  • eBook Packages: Springer Protocols

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