Abstract
High-speed atomic force microscopy (HS-AFM) with high spatiotemporal resolution allows for the video imaging of the conformational changes of individual molecules in an observation area in liquid at nanometer-scale spatial resolution. This method verifies the molecular mechanism and reveals the structural dynamics of relevant biomolecules for various biological phenomena. Here, we describe the methods for HS-AFM observation and the analysis of the structural dynamics of individual amyloidogenic protein assemblies using amyloid β 1–42 as an example.
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Acknowledgments
The authors would like to thank Prof. T. Ando, Prof. T. Uchihashi, Dr. N. Kodera, and Dr. H. Konno for the technical advice concerning our HS-AFM observations.
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Watanabe-Nakayama, T., Ono, K. (2018). High-Speed Atomic Force Microscopy of Individual Amyloidogenic Protein Assemblies. In: Lyubchenko, Y. (eds) Nanoscale Imaging. Methods in Molecular Biology, vol 1814. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8591-3_12
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DOI: https://doi.org/10.1007/978-1-4939-8591-3_12
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