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Study of IgE-Producing B Cells Using the Verigem Fluorescent Reporter Mouse

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Type 2 Immunity

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1799))

Abstract

Immunoglobulin E (IgE) is the least abundant antibody isotype in mammalians, yet it plays a critical role in allergy and asthma. IgE-producing (IgE+) B cells are rare and difficult to detect, which have hindered progress to understand their generation and differentiation. Recently developed new fluorescent IgE reporter mice have enabled better understanding of the biology of IgE+ B cells. We here describe the usage of the Verigem IgE reporter mouse to study IgE+ B cells and plasma cells by flow cytometry and microscopy.

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Acknowledgments

These protocols were developed for research projects supported by the UCSF Sandler Asthma Basic Research Center, the UCSF Cardiovascular Research Institute, the Weston Havens Foundation, and grants DP2HL117752 and R01AI103146 from the National Institutes of Health. C.D.C.A. is a Pew Scholar in the Biomedical Sciences, supported by The Pew Charitable Trusts. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health or of the Pew Charitable Trusts.

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Authors and Affiliations

  1. Cardiovascular Research Institute, University of California, San Francisco, CA, USA

    Zhiyong Yang, James B. Jung & Christopher D. C. Allen

  2. Sandler Asthma Basic Research Center, University of California, San Francisco, CA, USA

    Zhiyong Yang, James B. Jung & Christopher D. C. Allen

  3. Department of Anatomy, University of California, San Francisco, CA, USA

    Christopher D. C. Allen

Authors
  1. Zhiyong Yang
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  2. James B. Jung
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  3. Christopher D. C. Allen
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Corresponding author

Correspondence to Christopher D. C. Allen .

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Editors and Affiliations

  1. Department of Biomedical Research, National Jewish Health, Denver, CO, USA

    R. Lee Reinhardt

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Yang, Z., Jung, J.B., Allen, C.D.C. (2018). Study of IgE-Producing B Cells Using the Verigem Fluorescent Reporter Mouse. In: Reinhardt, R. (eds) Type 2 Immunity. Methods in Molecular Biology, vol 1799. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-7896-0_19

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  • DOI: https://doi.org/10.1007/978-1-4939-7896-0_19

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-7895-3

  • Online ISBN: 978-1-4939-7896-0

  • eBook Packages: Springer Protocols

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