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Type 2 Immunity

Methods and Protocols

  • Book
  • © 2018

Overview

Editors:
  1. R. Lee Reinhardt

    1. Department of Biomedical Research, National Jewish Health, Denver, USA

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1799)

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Table of contents (32 protocols)

  1. Front Matter

    Pages i-xvi
    Download chapter PDF

  2. A Fungal Protease Model to Interrogate Allergic Lung Immunity

    • J. Morgan Knight, Evan Li, Hui-Ying Tung, Cameron Landers, Jake Wheeler, Farrah Kheradmand et al.
    Pages 1-9

  3. Use of the Litomosoides sigmodontis Infection Model of Filariasis to Study Type 2 Immunity

    • A. Fulton, S. A. Babayan, M. D. Taylor
    Pages 11-26

  4. Production of Hymenolepis diminuta in the Laboratory: An Old Research Tool with New Clinical Applications

    • Min Zhang, Amanda J. Mathew, William Parker
    Pages 27-38

  5. A Mouse Model of Peanut Allergy Induced by Sensitization Through the Gastrointestinal Tract

    • Kelly Orgel, Michael Kulis
    Pages 39-47

  6. Induction and Characterization of the Allergic Eye Disease Mouse Model

    • Nancy J. Reyes, Rose Mathew, Daniel R. Saban
    Pages 49-57

  7. Isolation and Purification of Epithelial and Endothelial Cells from Mouse Lung

    • Hideki Nakano, Keiko Nakano, Donald N. Cook
    Pages 59-69

  8. In Vitro and In Vivo IgE-/Antigen-Mediated Mast Cell Activation

    • Hae Woong Choi, Soman N. Abraham
    Pages 71-80

  9. The Use of Human and Mouse Mast Cell and Basophil Cultures to Assess Type 2 Inflammation

    • Heather L. Caslin, Marcela T. Taruselli, Anuya Paranjape, Kasalina Kiwanuka, Tamara Haque, Alena P. Chumanevich et al.
    Pages 81-92

  10. Isolation and Identification of Group 2 Innate Lymphoid Cells in Settings of Type 2 Inflammation

    • Jesse Charles Nussbaum, Jorge Felipe Ortiz-Carpena
    Pages 93-107

  11. Determination of the Fate and Function of Innate Lymphoid Cells Following Adoptive Transfer of Innate Lymphoid Cell Precursors

    • Timothy E. O’Sullivan, Joseph C. Sun
    Pages 109-119

  12. Characterization of Thymic Development of Natural Killer T Cell Subsets by Multiparameter Flow Cytometry

    • Kathryn D. Tuttle, Laurent Gapin
    Pages 121-133

  13. Characterization of Mouse γδ T Cell Subsets in the Setting of Type-2 Immunity

    • Wanjiang Zeng, Rebecca L. O’Brien, Willi K. Born, Yafei Huang
    Pages 135-151

  14. The Identification of Allergen-Derived T Cell Epitopes

    • Véronique Schulten, Alessandro Sette
    Pages 153-163

  15. Generation of Allergen-Specific Tetramers for a Murine Model of Airway Inflammation

    • James J. Moon, Marion Pepper
    Pages 165-181

  16. The Generation and Use of Allergen-Specific TCR Transgenic Animals

    • Manon Vanheerswynghels, Wendy Toussaint, Martijn Schuijs, Leen Vanhoutte, Nigel Killeen, Hamida Hammad et al.
    Pages 183-210

  17. Using Cytokine Reporter Mice to Visualize Type-2 Immunity In Vivo

    • Mark Dell’Aringa, R. Lee Reinhardt
    Pages 211-223

  18. Live Imaging of IL-4-Expressing T Follicular Helper Cells in Explanted Lymph Nodes

    • Mark Dell’Aringa, R. Lee Reinhardt, Rachel S. Friedman, Jordan Jacobelli
    Pages 225-235

  19. Imaging Precision-Cut Lung Slices to Visualize Leukocyte Localization and Trafficking

    • Miranda R. Lyons-Cohen, Hideki Nakano, Seddon Y. Thomas, Donald N. Cook
    Pages 237-246

  20. Study of IgE-Producing B Cells Using the Verigem Fluorescent Reporter Mouse

    • Zhiyong Yang, James B. Jung, Christopher D. C. Allen
    Pages 247-264
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Keywords

About this book

This book provides researchers the opportunity to investigate type-2-associated diseases in their laboratories. Beginning with chapters describing various models of type-2 immunity, the volume then continues by detailing cellular protocols designed to identify, characterize, and assess the function of key adaptive and innate immune cells involved in type-2 inflammation; approaches to isolate and evaluate specific cellular subsets at the genetic, epigenetic, and molecular level; protocols to assess type-2 immunity and its relationship to organismal and metabolic systems (ex. Microbiome). This book concludes with a section that explores the use of primary human cells in evaluating relevance to the clinic. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshootingand avoiding known pitfalls.

Vital and authoritative, Type 2 Immunity: Methods and Protocols aims to provide a broad network of methods that can be used to develop a hypothesis and investigate its potential from bench to beside. 

Editors and Affiliations

  • Department of Biomedical Research, National Jewish Health, Denver, USA

    R. Lee Reinhardt

Bibliographic Information

  • Book Title: Type 2 Immunity

  • Book Subtitle: Methods and Protocols

  • Editors: R. Lee Reinhardt

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7896-0

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2018

  • Hardcover ISBN: 978-1-4939-7895-3Published: 29 June 2018

  • Softcover ISBN: 978-1-4939-9318-5Published: 10 December 2019

  • eBook ISBN: 978-1-4939-7896-0Published: 28 June 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XVI, 434

  • Number of Illustrations: 27 b/w illustrations, 53 illustrations in colour

  • Topics: Immunology

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