Abstract
Extracellular RNAs are initiating increased interest due to their potentials in serving as novel biomarkers, mediators of intercellular communication, and therapeutic applications. As a newly emerging field, one of the main obstacles is the lack of standardized protocols for RNA isolations. Here we describe protocols for commercially available kits that have been modified to yield consistent results for isolation of extracellular RNA from both whole serum/plasma and extracellular vesicle-enriched serum/plasma samples.
Justyna Filant, Parham Nejad, Anu Paul, Bridget Simonson, Srimeenakshi Srinivasan, Xuan Zhang, Leonora Balaj are Lab members (listed in alphabetical order).
Saumya Das, Roopali Gandhi, Louise C. Laurent, Anil K. Sood are Lab PI’s (listed in alphabetical order).
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Acknowledgements
J.F. and A.S. were supported by NIH grant UH3 TR000943. P.N., A.P., and R.G. were supported by UH2-UH3 TR000890. B.S. and S.D. were supported by UH3TR000901. S.S. and L.L. were supported by U01 HL126494 and UH3 TR000906. X.Z. and L.B. were supported by U19 CA179463. Christina Bennett helped in proofreading and formatting the article.
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Filant, J. et al. (2018). Isolation of Extracellular RNA from Serum/Plasma. In: Patel, T. (eds) Extracellular RNA. Methods in Molecular Biology, vol 1740. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7652-2_5
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DOI: https://doi.org/10.1007/978-1-4939-7652-2_5
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-7651-5
Online ISBN: 978-1-4939-7652-2
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