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Use of a Hollow Fiber Bioreactor to Collect Extracellular Vesicles from Cells in Culture

  • Irene K. Yan
  • Neha Shukla
  • David A. Borrelli
  • Tushar PatelEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1740)

Abstract

Current approaches for collection of extracellular vesicles (EV) are based on classical cell culture media production. This involves collection from cells grown in flasks, and can require multiple rounds of centrifugation or filtration, followed by ultracentrifugation or density gradient centrifugation. There are several limitations of these approaches, for example, they require a large input volume, the yield and concentration is low, and the process is time consuming. Most cell cultures require the use of fetal bovine serum which contains a large amount of endogenous EV that can contaminate isolations of cell-derived EVs. The use of cell cultures within a hollow fiber bioreactor could address many of these limitations and produce a continuous source of highly concentrated EVs without contamination from serum EVs, and that are suitable for downstream applications.

Keywords

Extracellular vesicles Isolation Hollow fiber bioreactor Exosomes 

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Copyright information

© Springer Science+Business Media, LLC 2018

Authors and Affiliations

  • Irene K. Yan
    • 1
  • Neha Shukla
    • 1
  • David A. Borrelli
    • 1
  • Tushar Patel
    • 1
    Email author
  1. 1.Department of TransplantationMayo ClinicJacksonvilleUSA

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