Isolation and Expansion of Schwann Cells from Transgenic Mouse Models
The most widely used method (Brockes’ method) for preparing primary Schwann cell culture uses neonatal rat sciatic nerves as the primary source of Schwann cells. The procedure is relatively simple and yields a highly purified population of Schwann cells in a short period of time. The method has also been used to prepare Schwann cells from mice, however, with limitation. For example, Brockes’ method is not applicable when the genotypes of mouse neonates are unknown or if the mouse mutants do not develop to term. We described a method ideal for preparing Schwann cells in a transgenic/knockout mouse study. The method uses embryonic dorsal root ganglia as the primary source of Schwann cells and allows preparing separate, highly purified Schwann cell cultures from individual mouse embryos in less than 2 weeks.
Key wordsMouse Schwann cells Mouse embryos Dorsal root ganglia
- 11.Ng A, Logan AM, Schmidt EJ, Robinson FL (2013) The CMT4B disease-causing phosphatases Mtmr2 and Mtmr13 localize to the Schwann cell cytoplasm and endomembrane compartments, where they depend upon each other to achieve wild-type levels of protein expression. Hum Mol Genet 22(8):1493–1506CrossRefPubMedPubMedCentralGoogle Scholar
- 12.Ratner N, Williams JP, Kordich JJ, Kim HA (2005) Schwann cell preparation from single mouse embryos: analyses of neurofibromin function in Schwann cells. Methods Enzymol 407:22–33Google Scholar
- 16.Miyamoto Y, Torii T, Kawahara K, Tanoue A, Yamauchi J (2016) Dock8 interacts with Nck1 in mediating Schwann cell precursor migration. Biochem Biophys Rep 6:113–123Google Scholar
- 20.Wu J, Liu W, Williams JP, Ratner N (2017) EGFR-Stat3 signalling in nerve glial cells modifies neurofibroma initiation. Oncogene 36:1669–1677Google Scholar