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Schwann Cells

Methods and Protocols

  • Paula V. Monje
  • Haesun A. Kim

Part of the Methods in Molecular Biology book series (MIMB, volume 1739)

Table of contents

  1. Front Matter
    Pages i-xiii
  2. Protocols for Preparing Primary Cultures, Cell Lines and Engineered Schwann Cells

    1. Front Matter
      Pages 1-1
    2. Rhona Mirsky, Kristjan R. Jessen
      Pages 3-15
    3. Tamara Weiss, Sabine Taschner-Mandl, Peter F. Ambros, Inge M. Ambros
      Pages 67-86
    4. Adriana E. Brooks, Gagani Athauda, Mary Bartlett Bunge, Aisha Khan
      Pages 111-126
    5. Y. P. Tsui, Graham K. Shea, Y. S. Chan, Daisy K. Y. Shum
      Pages 137-148
  3. Protocols for Studying Schwann Cell Behavior and Myelination In Vitro

    1. Front Matter
      Pages 175-175
    2. Vania W. Almeida, Margaret L. Bates, Mary Bartlett Bunge
      Pages 195-212
    3. Susana R. Cerqueira, Yee-Shuan Lee, Mary Bartlett Bunge
      Pages 269-279
    4. Cristian De Gregorio, Paula Díaz, Rodrigo López-Leal, Patricio Manque, Felipe A. Court
      Pages 299-315
    5. Laura Fangmann, Steffen Teller, Pavel Stupakov, Helmut Friess, Güralp O. Ceyhan, Ihsan Ekin Demir
      Pages 317-325
  4. Protocols for Studying Schwann Cell Development and Myelination In Vivo

  5. Protocols for Schwann Cell Transplantation

    1. Front Matter
      Pages 407-407
    2. Christine D. Plant, Giles W. Plant
      Pages 439-453
    3. Christine D. Plant, Giles W. Plant
      Pages 455-466
    4. Andrea J. Santamaría, Juan P. Solano, Francisco D. Benavides, James D. Guest
      Pages 467-484
  6. Back Matter
    Pages 485-488

About this book

Introduction

This volume presents an assortment of traditional and emerging experimental procedures relevant to Schwann cell research. The chapters are divided into four parts. Part I contains protocols for in vitro culture, purification, and characterization of primary Schwann cells from diverse species and stages of nerve development. It also contains protocols to create cancer cell lines and engineered Schwann cells from unconventional sources via chemical conversion, induced differentiation or genetic intervention. Parts II and III outline a wide range of methodologies used to study Schwann cells within in vitro and in vivo systems relevant to the analysis of peripheral nerve development, cancer, axon degeneration/regeneration, and myelination. Last but not least, part IV outlines protocols for Schwann cell production, collection, labeling and transplantation in the injured peripheral nerve and spinal cord of experimental animals and human subjects.

Authoritative and practical, Schwann Cells: Methods and Protocols aims to aid both experienced and new investigators to make progress in their research endeavors involving Schwann cells.

Keywords

Cell culture Genetic manipulation Imaging techniques Cell differentiation Cell interactions

Editors and affiliations

  • Paula V. Monje
    • 1
  • Haesun A. Kim
    • 2
  1. 1.Department of Neurological SurgeryUniversity of MiamiMiamiUSA
  2. 2.Department of Biological SciencesRutgers UniversityNewarkUSA

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4939-7649-2
  • Copyright Information Springer Science+Business Media, LLC 2018
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-7648-5
  • Online ISBN 978-1-4939-7649-2
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site