Abstract
It has increasingly been considered crucial the understanding of DNA methylation of Tumor Suppressor Gene (TSG) promoters, such as that of retinoblastoma 1 gene (RB1), and its role during carcinogenesis. We present a detailed and optimized protocol of the methylation-specific PCR (MSP) technique to study RB1 gene promoter hypermethylation.
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McCormick, T.M., Carvalho, M.D.G.D.C. (2018). Using Methylation-Specific PCR to Study RB1 Promoter Hypermethylation. In: Santiago-Cardona, P. (eds) The Retinoblastoma Protein. Methods in Molecular Biology, vol 1726. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7565-5_4
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DOI: https://doi.org/10.1007/978-1-4939-7565-5_4
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-7564-8
Online ISBN: 978-1-4939-7565-5
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