Abstract
Human transcriptome contains a large number of circular RNAs (circRNAs) that are mainly produced by back splicing of pre-mRNA. Here we describe a minigene reporter system containing a single exon encoding split GFP in reverse order, which can be efficiently back spliced to produce a circRNA encoding intact GFP gene. This simple reporter system can be adopted to study how different cis-elements and trans-factors affect circRNA production, and also can serve as a reliable system to measure the activity of IRES-mediated translation. Therefore this system can serve as a platform for mechanistic studies on the circRNA biogenesis and its function.
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Yang, Y., Wang, Z. (2018). Constructing GFP-Based Reporter to Study Back Splicing and Translation of Circular RNA. In: Dieterich, C., Papantonis, A. (eds) Circular RNAs. Methods in Molecular Biology, vol 1724. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7562-4_9
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DOI: https://doi.org/10.1007/978-1-4939-7562-4_9
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-7561-7
Online ISBN: 978-1-4939-7562-4
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