Abstract
A wide variety of bioactive peptides are present in all metazoan species where they govern diverse functions as small messenger molecules. In the last 15 years, mass spectrometry-based methods have identified endogenous peptides in diverse species. Mass spectrometry enables the precise peptide sequences to be determined, including the potential existence of truncated versions or the presence of post-translational modifications. Because small modifications can have a large effect on biological activity, knowledge of the actual peptide sequences paves the way for further functional studies such as analysis of neuropeptidergic signaling cascades. Zebrafish (Danio rerio) is an important animal model that is commonly used in a wide range of studies. Here we provide a detailed description of the peptide extraction procedure and peptidomics workflow for zebrafish.
Funding
The authors highly appreciate funding from the University Research Fund (Bijzonder Onderzoeksfonds, BOF).
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Cerda-Reverter JM, Agulleiro MJ, RG R, Sanchez E, Ceinos R, Rotllant J (2011) Fish melanocortin system. Eur J Pharmacol 660:53–60
Sherwood NM, Adams BA, Isaac ER, Wu S, Fradinger EA (2007) Knocked down and out: PACAP in development, reproduction and feeding. Peptides 28:1680–1687
Matsuda K, Sakashita A, Yokobori E, Azuma M (2012) Neuroendocrine control of feeding behavior and psychomotor activity by neuropeptide Y in fish. Neuropeptides 46:275–283
Lowry CA, Moore FL (2006) Regulation of behavioral responses by corticotropin-releasing factor. Gen Comp Endocrinol 146:19–27
Panula P (2010) Hypocretin/orexin in fish physiology with emphasis on zebrafish. Acta Physiol (Oxf) 198:381–386
Husson SJ, Clynen E, Baggerman G, De Loof A, Schoofs L (2005) Discovering neuropeptides in Caenorhabditis elegans by two dimensional liquid chromatography and mass spectrometry. Biochem Biophys Res Commun 335:76–86
Husson SJ, Landuyt B, Nys T, Baggerman G, Boonen K, Clynen E, Lindemans M, Janssen T, Schoofs L (2009) Comparative peptidomics of Caenorhabditis elegans versus C. briggsae by LC—MALDI-TOF MS. Peptides 30:449–457
Baggerman G, Cerstiaens A, De Loof A, Schoofs L (2002) Peptidomics of the larval Drosophila melanogaster central nervous system. J Biol Chem 277:40368–40374
Van Camp KA, Baggerman G, Blust R, Husson SJ (2017) Peptidomics of the zebrafish Danio rerio: in search for neuropeptides. J Proteomics 150:290–296
Maes E, Valkenborg D, Baggerman G, Willems H, Landuyt B, Schoofs L, Mertens I (2015) Determination of variation parameters as a crucial step in designing TMT-based clinical proteomics experiments. PLoS One 10:1–15
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2018 Springer Science+Business Media, LLC
About this protocol
Cite this protocol
Van Camp, K.A., Baggerman, G., Blust, R., Husson, S.J. (2018). Neuropeptidomic Analysis of Zebrafish Brain. In: Schrader, M., Fricker, L. (eds) Peptidomics. Methods in Molecular Biology, vol 1719. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7537-2_16
Download citation
DOI: https://doi.org/10.1007/978-1-4939-7537-2_16
Published:
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-7536-5
Online ISBN: 978-1-4939-7537-2
eBook Packages: Springer Protocols