Abstract
The isolation of thylakoid membranes, including intact membrane protein complexes, from heterocysts of filamentous cyanobacteria such as Nostoc punctiforme, is described. Protocols for BN-PAGE/SDS-PAGE 2-D electrophoresis are not included. However, the chapter ends with advisory notes on sample preparation for blue-native PAGE of thylakoid membrane proteins, which can then be used together with any standard protocol.
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References
Muro-Pastor AM, Hess WR (2012) Heterocyst differentiation: from single mutants to global approaches. Trends Microbiol 20:548–557
Curatti L, Flores E, Salerno G (2002) Sucrose is involved in the diazotrophic metabolism of the heterocyst-forming cyanobacterium Anabaena sp. FEBS Lett 513:175–178
López-Igual R, Flores E, Herrero A (2010) Inactivation of a heterocyst specific invertase indicates a principal role of sucrose catabolism in the heterocysts of Anabaena sp. J Bacteriol 192:5526–5533
Magnuson A, Cardona T (2016) Thylakoid membrane function in heterocysts. BBA-Bioenergetics 1857:309–319
Cardona T, Battchikova N, Zhang PP, Stensjö K, Aro EM, Lindblad P, Magnuson A (2009) Electron transfer protein complexes in the thylakoid membranes of heterocysts from the cyanobacterium Nostoc punctiforme. BBA-Bioenergetics 1787:252–263
Simpson RJ (2010) Disruption of cultured cells by nitrogen cavitation, Cold Spring Harb Protoc 2010. doi:10.1101/pdb.prot5513
Rippka R, Deruelles J, Waterbury JB, Herdman M, Stonier RY (1979) Generic assignments, strain histories and properties of pure cultures of cyanobacteria. J Gen Microbiol 111:1–61
Meeks J, Castenholtz R (1971) Growth and photosynthesis in an extreme thermophile, Synechococcus lividus (Cyanophyta). Arch Mikrobiol 78:25–41
Schägger H, vonJagow G (1987) Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. Anal Biochem 166:368–379
Schägger H, vonJagow G (1991) Blue native electrophoresis for isolation of membrane protein complexes in ezymatically active form. Anal Biochem 199:223–231
Wittig I, Braun HP, Schägger H (2007) Blue native PAGE. Nat Protoc 1:418–428
Prommeenate P, Lennon AM, Markert C, Hippler M, Nixon PJ (2004) Subunit composition of NDH-1 complexes of Synechocystis sp. PCC 6803. J Biol Chem 279:28165–28173
Danielsson R, Suorsa M, Paakkarinen V, Albertsson PA, Styring S, Aro EM, Mamedov M (2006) Dimeric and monomeric organization of photosystem II – distribution of five distinct complexes in the different domains of the thylakoid membrane. J Biol Chem 281:14241–14249
Watanabe M, Kubota H, Wada H, Narikawa R, Ikeuchi M (2010) Novel supercomplex organization of photosystem I in Anabaena and Cyanophora paradoxa. Plant Cell Physiol 52:162–168
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Magnuson, A., Cardona, T. (2018). Isolation of Intact Thylakoid Membranes from Heterocysts of Filamentous, Nitrogen-Fixing Cyanobacteria. In: Mock, HP., Matros, A., Witzel, K. (eds) Plant Membrane Proteomics. Methods in Molecular Biology, vol 1696. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7411-5_9
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DOI: https://doi.org/10.1007/978-1-4939-7411-5_9
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