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Abiotic Stress Phenotyping of Polyamine Mutants

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Polyamines

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1694))

Abstract

Plant mutants in polyamine pathway genes are ideal for investigating their roles in stress responses. Here we describe easy-to-perform methods for phenotyping Arabidopsis mutants under abiotic stress. These include measurements of root growth, chlorophyll content, water loss, electrolyte leakage, and content of the reactive oxygen species hydrogen peroxide (H2O2) and superoxide anion (O2−). Growth of Arabidopsis seedlings is described that enables transfer to different media for stress treatment without damaging roots.

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Acknowledgements

This work is financially supported by JSPS KAKENHI (No. 15K14705) to T.K.

Author information

Authors and Affiliations

  1. Laboratory Center, Senckenberg Biodiversity and Climate Research Center (SBiK-F), Georg-Voigt-Str. 14-16, 60325, Frankfurt am Main, Germany

    Thomas Berberich

  2. Department of Genetics & Plant Breeding, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh

    G. H. M. Sagor

  3. Graduate School of Life Sciences, Tohoku University, 2-1-1 Katahira, Aoba, Sendai, Miyagi, 980-8577, Japan

    Tomonobu Kusano

Authors
  1. Thomas Berberich
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  2. G. H. M. Sagor
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  3. Tomonobu Kusano
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Corresponding author

Correspondence to Thomas Berberich .

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Editors and Affiliations

  1. Department of Biology, Healthcare and Environment, Faculty of Pharmacy and Food Sciences, Section of Plant Physiology, University of Barcelona, Spain

    Rubén Alcázar

  2. Department of Biology, Healthcare and Environment, Faculty of Pharmacy and Food Sciences, Section of Plant Physiology, University of Barcelona, Spain

    Antonio F. Tiburcio

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Berberich, T., Sagor, G.H.M., Kusano, T. (2018). Abiotic Stress Phenotyping of Polyamine Mutants. In: Alcázar, R., Tiburcio, A. (eds) Polyamines. Methods in Molecular Biology, vol 1694. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7398-9_32

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  • DOI: https://doi.org/10.1007/978-1-4939-7398-9_32

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7397-2

  • Online ISBN: 978-1-4939-7398-9

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