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Identification of Acetylated Proteins in Borrelia burgdorferi

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Borrelia burgdorferi

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1690))

Abstract

Posttranslational modification (PTM) of proteins has emerged as a major regulatory mechanism in all three domains of life. One emerging PTM is Nε-lysine acetylation—the acetylation of the epsilon amino group of lysine residues. Nε-lysine acetylation is known to regulate multiple cellular processes. In eukaryotes, it regulates chromatin structure, transcription, metabolism, signal transduction, and the cytoskeleton. Recently, multiple groups have detected Nε-lysine acetylation in diverse bacterial phyla, but no work on protein acetylation in Borrelia burgdorferi has been reported. Here, we describe a step-by-step protocol to identify Nε-lysine acetylated proteins in B. burgdorferi.

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Acknowledgments

This work was supported by grants from the HHS | NIH | National Institute of Allergy and Infectious Diseases (NIAID) (AI4684064 to XFY).

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Authors and Affiliations

  1. Department of Microbiology and Immunology, Indiana University School of Medicine, 635 Barnhill Drive, Indianapolis, IN, 46202, USA

    Youyun Yang & X. Frank Yang

  2. Department of Microbiology and Immunology, Loyola University Chicago, Stritch School of Medicine, Maywood, IL, USA

    Alan Wolfe

Authors
  1. Youyun Yang
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  2. Alan Wolfe
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  3. X. Frank Yang
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Corresponding author

Correspondence to X. Frank Yang .

Editor information

Editors and Affiliations

  1. Department of Veterinary Medicine, University of Maryland, College Park, Maryland, USA

    Utpal Pal

  2. Department of Microbiology, Ondokuz Mayis University, Samsun, Turkey

    Ozlem Buyuktanir

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Yang, Y., Wolfe, A., Yang, X.F. (2018). Identification of Acetylated Proteins in Borrelia burgdorferi . In: Pal, U., Buyuktanir, O. (eds) Borrelia burgdorferi. Methods in Molecular Biology, vol 1690. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7383-5_14

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  • DOI: https://doi.org/10.1007/978-1-4939-7383-5_14

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7382-8

  • Online ISBN: 978-1-4939-7383-5

  • eBook Packages: Springer Protocols

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