Abstract
Chromatin immunoprecipitation is used to measure the binding of transcription factors to target DNA sequences in order to better understand transcriptional regulation. Here, we describe a process to analyze bacterial transcription factor binding in the context of an infected eukaryotic host cell. Using this approach, we measured the binding kinetics of three Chlamydia trachomatis transcription factors within infected cells, and demonstrated temporal changes in binding.
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Hanson, B.R., Tan, M. (2018). Using Intra-ChIP to Measure Protein–DNA Interactions in Intracellular Pathogens. In: Visa, N., Jordán-Pla, A. (eds) Chromatin Immunoprecipitation. Methods in Molecular Biology, vol 1689. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7380-4_13
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DOI: https://doi.org/10.1007/978-1-4939-7380-4_13
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-7379-8
Online ISBN: 978-1-4939-7380-4
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