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Liquid Chromatography/Mass Spectrometry (LC/MS) for the Detection and Quantification of N-Acyl-L-Homoserine Lactones (AHLs) and 4-Hydroxy-2-Alkylquinolines (HAQs)

  • François Lépine
  • Sylvain Milot
  • Marie-Christine Groleau
  • Eric Déziel
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1673)

Abstract

High-performance liquid chromatography (HPLC) coupled in-line with mass spectrometry (MS) permits rapid and specific identification and quantification of N-acyl-L-homoserine lactones (AHLs) and 4-hydroxy-2-alkylquinolines (HAQs). We are presenting here methods for the analysis of these molecules directly from biological samples using LC/MS.

Key words

Bacteria Quorum sensing Acyl-homoserine lactone 4-Hydroxy-2-alkylquinoline Analysis Quantification Mass spectrometry Liquid chromatography 

Notes

Acknowledgments

We thank Émilie Gauthier for the help in the development of the AHL quantification method. Work on AHLs and HAQs in the Déziel lab is funded by the Canadian Institutes of Health Research (CIHR). ED holds the Canada Research Chair in Sociomicrobiology.

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Copyright information

© Springer Science+Business Media LLC 2018

Authors and Affiliations

  • François Lépine
    • 1
  • Sylvain Milot
    • 1
  • Marie-Christine Groleau
    • 1
  • Eric Déziel
    • 1
  1. 1.INRS-Institut Armand-FrappierLavalCanada

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