Abstract
High-performance liquid chromatography (HPLC) coupled in-line with mass spectrometry (MS) permits rapid and specific identification and quantification of N-acyl-L-homoserine lactones (AHLs) and 4-hydroxy-2-alkylquinolines (HAQs). We are presenting here methods for the analysis of these molecules directly from biological samples using LC/MS.
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References
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Acknowledgments
We thank Émilie Gauthier for the help in the development of the AHL quantification method. Work on AHLs and HAQs in the Déziel lab is funded by the Canadian Institutes of Health Research (CIHR). ED holds the Canada Research Chair in Sociomicrobiology.
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Lépine, F., Milot, S., Groleau, MC., Déziel, E. (2018). Liquid Chromatography/Mass Spectrometry (LC/MS) for the Detection and Quantification of N-Acyl-L-Homoserine Lactones (AHLs) and 4-Hydroxy-2-Alkylquinolines (HAQs). In: Leoni, L., Rampioni, G. (eds) Quorum Sensing. Methods in Molecular Biology, vol 1673. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7309-5_4
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DOI: https://doi.org/10.1007/978-1-4939-7309-5_4
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