Liquid Chromatography/Mass Spectrometry (LC/MS) for the Detection and Quantification of N-Acyl-L-Homoserine Lactones (AHLs) and 4-Hydroxy-2-Alkylquinolines (HAQs)
High-performance liquid chromatography (HPLC) coupled in-line with mass spectrometry (MS) permits rapid and specific identification and quantification of N-acyl-L-homoserine lactones (AHLs) and 4-hydroxy-2-alkylquinolines (HAQs). We are presenting here methods for the analysis of these molecules directly from biological samples using LC/MS.
Key wordsBacteria Quorum sensing Acyl-homoserine lactone 4-Hydroxy-2-alkylquinoline Analysis Quantification Mass spectrometry Liquid chromatography
We thank Émilie Gauthier for the help in the development of the AHL quantification method. Work on AHLs and HAQs in the Déziel lab is funded by the Canadian Institutes of Health Research (CIHR). ED holds the Canada Research Chair in Sociomicrobiology.
- 3.Déziel E, Lépine F, Milot S, He J, Mindrinos MN, Tompkins RG et al (2004) Analysis of Pseudomonas aeruginosa 4-hydroxy-2-alkylquinolines (HAQs) reveals a role for 4-hydroxy-2-heptylquinoline in cell-to-cell communication. Proc Natl Acad Sci U S A 101:1339–1344CrossRefPubMedPubMedCentralGoogle Scholar
- 6.Vial L, Lépine F, Milot S, Groleau MC, Dekimpe V, Woods DE et al (2008) Burkholderia pseudomallei, B. thailandensis, and B. ambifaria produce 4-hydroxy-2-alkylquinoline analogues with a methyl group at the 3 position that is required for quorum-sensing regulation. J Bacteriol 190:5339–5352CrossRefPubMedPubMedCentralGoogle Scholar