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Visualizing Virus-Derived dsRNA Using Antibody-Independent and -Dependent Methods

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Innate Antiviral Immunity

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1656))

Abstract

Long double-stranded (ds) RNA molecules are produced as a byproduct of viral replication. Studying virus-derived dsRNA is important for understanding virus replication, understanding host responses to virus infections, and as a diagnostic tool for virus presence and replication. Here, we describe four different techniques for visualizing dsRNA; two antibody-dependent methods (immunoblotting and immunocytochemistry), as well as two antibody-independent methods (differential digestion and acridine orange staining). The benefits and disadvantages of each technique are also discussed.

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Authors and Affiliations

  1. Department of Biology, Wilfrid Laurier University, Waterloo, ON, Canada, N2L 3G1

    Sarah J. Poynter

  2. Departments of Health Sciences and Biology, Wilfrid Laurier University, Waterloo, ON, Canada

    Stephanie J. DeWitte-Orr

Authors
  1. Sarah J. Poynter
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  2. Stephanie J. DeWitte-Orr
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Corresponding author

Correspondence to Stephanie J. DeWitte-Orr .

Editor information

Editors and Affiliations

  1. Biochemistry and Biomedical Sciences, McMaster University, Hamilton, ON, Canada

    Karen Mossman

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Poynter, S.J., DeWitte-Orr, S.J. (2017). Visualizing Virus-Derived dsRNA Using Antibody-Independent and -Dependent Methods. In: Mossman, K. (eds) Innate Antiviral Immunity. Methods in Molecular Biology, vol 1656. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7237-1_5

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  • DOI: https://doi.org/10.1007/978-1-4939-7237-1_5

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7236-4

  • Online ISBN: 978-1-4939-7237-1

  • eBook Packages: Springer Protocols

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