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Innate Antiviral Immunity

Methods and Protocols

  • Karen Mossman

Part of the Methods in Molecular Biology book series (MIMB, volume 1656)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Fatemeh Vahedi, Elizabeth C. Giles, Ali A. Ashkar
    Pages 1-56
  3. Chandra S. Mukhopadhyay, Ramneek Verma, Jasdeep Singh
    Pages 89-102
  4. Sarah J. Poynter, Stephanie J. DeWitte-Orr
    Pages 103-118
  5. Renee C. Ireton, Courtney Wilkins, Michael Gale Jr.
    Pages 119-129
  6. Christine Vazquez, Dia C. Beachboard, Stacy M. Horner
    Pages 131-142
  7. Alice Mayer, Jonathan Maelfait, Anne Bridgeman, Jan Rehwinkel
    Pages 143-152 Open Access
  8. Vladislav Pokatayev, Nan Yan
    Pages 167-174
  9. Bryan J. Gall, Victor R. DeFilippis
    Pages 183-193
  10. Gabriel A. Leiva-Torres, Nestor Nebesio, Silvia M. Vidal
    Pages 209-227
  11. Sarah Katzenell, Jorge R. Cabrera, Brian J. North, David A. Leib
    Pages 229-251
  12. Claudia U. Duerr, Jörg H. Fritz
    Pages 253-261
  13. Biao Wang, Mark Loeb
    Pages 263-271
  14. Alice Mayer, Jonathan Maelfait, Anne Bridgeman, Jan Rehwinkel
    Pages E1-E1
  15. Back Matter
    Pages 273-275

About this book

Introduction

This book explores methods to study the complex and evolving interplay between a virus and its host that range from model systems to the detection of chemical molecules. The collection starts with the application of humanized mice and zebrafish as model organisms to study virus-host interactions and induction of innate immune responses. Subsequent chapters outline diverse methods to detect small interfering RNAs, microRNAs, and virus-derived dsRNA from a variety of cells, tissues, and organisms, as well as to interrogating the cytosolic RNA and DNA sensing pathways, including using RNA PAMPs as molecular tools, purification of cGAMP from virus particles and infected cells, and mechanisms to visualize the subcellular localization and activation of the adaptor proteins MAVS and STING. Cutting-edge methods, including high-throughput and genome-wide CRISPR/Cas9 screens, chromosome conformation capture, and whole-exome sequencing, are described to identify novel mediators, pathways, and variants underlying host susceptibility. Given the importance of studying these pathways and players under physiologic conditions, methods describing the isolation of primary mouse sensory neurons and group 2 innate lymphoid cells are also provided. Finally, this collection comes full circle back to the whole organism level and concludes with epidemiological methods to investigate virus-host interactions and the induction of innate immunity. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Innate Antiviral Immunity: Methods and Protocols spans a diverse array of approaches to study and elucidate the intricacies of this vital area of study.

Keywords

Virus-host interactions Model organisms Infected cells Novel pathways CRISPR

Editors and affiliations

  • Karen Mossman
    • 1
  1. 1.Biochemistry and Biomedical SciencesMcMaster UniversityHamiltonCanada

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4939-7237-1
  • Copyright Information Springer Science+Business Media LLC 2017
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-7236-4
  • Online ISBN 978-1-4939-7237-1
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site