Abstract
Cultured primary neurons have been of extraordinary value for the study of neuronal anatomy, cell biology, and physiology. While use of neuronal cell lines has ease and utility, there are often caveats that arise due to their mitotic nature. This methods article presents detailed methodology for the preparation, purification, and culture of adult murine sensory neurons for the study of herpes simplex virus lytic and latent infections. While virology is the application for our laboratory, these cultures also have broad utility for neurobiologists and cell biologists. While these primary cultures have been highly informative, the methodology is challenging to many investigators. Through publication of this highly detailed protocol, it is our hope that the use of this culture system can spread in the field to allow more rapid progress in furthering our understanding of neurotropic virus infection.
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Change history
23 October 2018
In chapter 15, section 2.3, the unit “180 μg/ml mouse laminin in HBSS. Prepare 150 μl per coverslip” is corrected to “18 μg/ml mouse laminin in HBSS. Prepare 150 μl per coverslip.”
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Acknowledgments
Development of the protocols described in this article was supported by PO1 AI098681, and by a pilot grant to JR Cabrera from the Hitchcock Foundation.
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Katzenell, S., Cabrera, J.R., North, B.J., Leib, D.A. (2017). Isolation, Purification, and Culture of Primary Murine Sensory Neurons. In: Mossman, K. (eds) Innate Antiviral Immunity. Methods in Molecular Biology, vol 1656. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7237-1_15
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DOI: https://doi.org/10.1007/978-1-4939-7237-1_15
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Publisher Name: Humana Press, New York, NY
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Online ISBN: 978-1-4939-7237-1
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