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Initial Considerations Before Designing a Promoter Construct

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Mammalian Synthetic Promoters

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1651))

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Abstract

Before designing a synthetic promoter, it can be helpful to think about its final application. Is the study purely an in vitro exercise in monitoring short-term promoter activity from an episomal vector, or does the promoter eventually need to be permanently active and be integrated into the genome or perhaps even to function in vivo? The final application will have a bearing on promoter design and vector of choice from the start of the study. In this chapter I highlight some of the vector attributes to consider and features that should be thought about.

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Authors and Affiliations

  1. Department of Biochemical Pharmacology, William Harvey Research Institute, Queen Mary University of London, London, EC1M 6BQ, UK

    David Gould

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  1. David Gould
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Correspondence to David Gould .

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  1. Department of Biochemical Pharmacology, William Harvey Research Institute, Queen Mary University of London, London, UK

    David Gould

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Gould, D. (2017). Initial Considerations Before Designing a Promoter Construct. In: Gould, D. (eds) Mammalian Synthetic Promoters. Methods in Molecular Biology, vol 1651. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-7223-4_1

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  • DOI: https://doi.org/10.1007/978-1-4939-7223-4_1

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-7221-0

  • Online ISBN: 978-1-4939-7223-4

  • eBook Packages: Springer Protocols

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