Abstract
Ebola virus (EBOV) replicates in host cells, where both viral and cellular components show morphological changes during the process of viral replication from entry to budding. These steps in the replication cycle can be studied using electron microscopy (EM), including transmission electron microscopy (TEM) and scanning electron microscopy (SEM), which is one of the most useful methods for visualizing EBOV particles and EBOV-infected cells at the ultrastructural level. This chapter describes conventional methods for EM sample preparation of cultured cells infected with EBOV.
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References
Geisbert TW, Jahrling PB (1995) Differentiation of filoviruses by electron microscopy. Virus Res 39:129–150
Noda T, Ebihara H, Muramoto Y et al (2006) Assembly and budding of Ebolavirus. PLoS Pathog 2:e99
Ellis DS, Simpson DIH, Francis DP et al (1978) Ultrastructure of Ebola virus particles in human liver. J Clin Pathol 31:201–208
Ellis DS, Stamford S, Lloyd G et al (1979) Ebola and Marburg viruses: I. Some ultrastructural differences between strains when grown in Vero cells. J Med Virol 4:201–211
Baskerville A, Fisher-Hoch SP, Neild GH et al (1985) Ultrastructural pathology of experimental Ebola hemorrhagic fever virus infection. J Pathol 147:199–209
Watanabe S, Watanabe T, Noda T et al (2005) Production of novel Ebola virus-like particles from cDNAs: an alternative to Ebola virus generation by reverse genetics. J Virol 78:999–1005
Neumann G, Ebihara H, Takada A et al (2005) Ebola virus VP40 late domains are not essential for viral replication in cell culture. J Virol 79:10300–10307
Halfmann O, Kim JH, Ebihara H et al (2008) Generation of biologically contained Ebola viruses. Proc Natl Acad Sci U S A 105:1129–1133
Acknowledgments
This work is supported by JST PRESTO and by the Research Program on Emerging and Re-emerging Infectious Diseases from the Japan Agency for Medical Research and Development.
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Noda, T. (2017). Electron Microscopy of Ebola Virus-Infected Cells. In: Hoenen, T., Groseth, A. (eds) Ebolaviruses. Methods in Molecular Biology, vol 1628. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7116-9_19
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DOI: https://doi.org/10.1007/978-1-4939-7116-9_19
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Publisher Name: Humana Press, New York, NY
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Online ISBN: 978-1-4939-7116-9
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