Methods and Protocols

  • Thomas Hoenen
  • Allison Groseth

Part of the Methods in Molecular Biology book series (MIMB, volume 1628)

Table of contents

  1. Front Matter
    Pages i-xvi
  2. Introduction

  3. Studying Ebolaviruses Under Biosafety Level 2 Conditions

    1. Front Matter
      Pages 51-51
    2. Patrick L. Sinn, Jeremy E. Coffin, Natarajan Ayithan, Kathleen H. Holt, Wendy Maury
      Pages 65-78
    3. Tessa Cressey, Kristina Brauburger, Elke Mühlberger
      Pages 79-92
    4. Olivier Reynard, Mathieu Mateo
      Pages 109-117
    5. Kristina Brauburger, Tessa Cressey, Elke Mühlberger
      Pages 143-159
  4. Studying Infectious Ebolaviruses In Vitro

    1. Front Matter
      Pages 161-161
    2. Claire Marie Filone, David Miller, Victoria Wahl-Jensen
      Pages 163-175
    3. Gordian Schudt, Olga Dolnik, Stephan Becker
      Pages 195-202

About this book


This vital book collects key methods that have supported advancements in the field of ebolavirus molecular biology given the pressing need for the advancement of techniques for diagnostics, the development of vaccines and antivirals, and for furthering our understanding of ebolavirus biology.  After an introduction, the volume delves into protocols for studying ebolavirus molecular biology under biosafety level 2 conditions, studying infectious ebolaviruses under biosafety level 4 conditions in vitro as well as in vivo, and working with ebolaviruses in the field.  Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Ebolaviruses: Methods and Protocols serves as a guide to the next generations of filovirus researchers and also helps to bring experts from other areas into the filovirus research arena.


Ebola virus Virology Zaire ebolavirus Biosafety levels Filoviruses Immune response

Editors and affiliations

  • Thomas Hoenen
    • 1
  • Allison Groseth
    • 2
  1. 1.Friedrich-Loeffler-InstitutGreifswald - Insel RiemsGermany
  2. 2.Friedrich-Loeffler-InstitutGreifswald - Insel RiemsGermany

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4939-7116-9
  • Copyright Information Springer Science+Business Media LLC 2017
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-7115-2
  • Online ISBN 978-1-4939-7116-9
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book