Abstract
In this chapter, we describe a method for the induction of stomach organoids from mouse embryonic stem (ES) cells. We used an embryoid body-based differentiation method to induce gastric primordial epithelium covered with mesenchyme and further differentiate it in Matrigel by 3D culture. The differentiated organoid contains both corpus- and antrum-specific mature gastric tissue cells. This protocol may be useful for a variety of studies in developmental biology and disease modeling of the stomach.
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Noguchi, Ta.K., Kurisaki, A. (2017). Formation of Stomach Tissue by Organoid Culture Using Mouse Embryonic Stem Cells. In: Tsuji, T. (eds) Organ Regeneration. Methods in Molecular Biology, vol 1597. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6949-4_16
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DOI: https://doi.org/10.1007/978-1-4939-6949-4_16
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-6947-0
Online ISBN: 978-1-4939-6949-4
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