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Formation of Stomach Tissue by Organoid Culture Using Mouse Embryonic Stem Cells

Part of the Methods in Molecular Biology book series (MIMB,volume 1597)

Abstract

In this chapter, we describe a method for the induction of stomach organoids from mouse embryonic stem (ES) cells. We used an embryoid body-based differentiation method to induce gastric primordial epithelium covered with mesenchyme and further differentiate it in Matrigel by 3D culture. The differentiated organoid contains both corpus- and antrum-specific mature gastric tissue cells. This protocol may be useful for a variety of studies in developmental biology and disease modeling of the stomach.

Key words

  • Stomach organoids
  • Embryonic stem cells
  • Tissue formation
  • Disease model
  • Differentiation
  • Shh
  • Dkk-1
  • Barx1

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  • DOI: 10.1007/978-1-4939-6949-4_16
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Correspondence to Taka-aki K. Noguchi or Akira Kurisaki .

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Noguchi, Ta.K., Kurisaki, A. (2017). Formation of Stomach Tissue by Organoid Culture Using Mouse Embryonic Stem Cells. In: Tsuji, T. (eds) Organ Regeneration. Methods in Molecular Biology, vol 1597. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6949-4_16

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  • DOI: https://doi.org/10.1007/978-1-4939-6949-4_16

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6947-0

  • Online ISBN: 978-1-4939-6949-4

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