Abstract
T cell antigen receptor (TCR) stimulation induces recruitment and accumulation of various types of signaling molecules and forms signaling microclusters. The dynamics of the microclusters are important for regulating the quality and quantity of T cell activation. We describe here our protocols for analysis of signaling microclusters by using supported planar bilayers.
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Acknowledgment
This work was supported by KAKENHI (Grant-in-Aid for Scientific Research (S) 24229004 for T.S. and (C) 10415226 for A.H.-T.), and partly by Takeda Science Foundation, Novartis Foundation (Japan), and Hayashi Memorial Foundation for Female Natural Scientists.
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Hashimoto-Tane, A., Yokosuka, T., Saito, T. (2017). Analyzing the Dynamics of Signaling Microclusters. In: Baldari, C., Dustin, M. (eds) The Immune Synapse. Methods in Molecular Biology, vol 1584. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6881-7_4
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DOI: https://doi.org/10.1007/978-1-4939-6881-7_4
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