The Immune Synapse

Methods and Protocols

  • Cosima T. Baldari
  • Michael L. Dustin

Part of the Methods in Molecular Biology book series (MIMB, volume 1584)

Table of contents

  1. Front Matter
    Pages i-xiii
  2. Michael L. Dustin, Cosima T. Baldari
    Pages 1-5
  3. Katarzyna I. Jankowska, Janis K. Burkhardt
    Pages 7-29
  4. Noelia Blas-Rus, Eugenio Bustos-Morán, Francisco Sánchez-Madrid, Noa B. Martín-Cófreces
    Pages 31-49
  5. Akiko Hashimoto-Tane, Tadashi Yokosuka, Takashi Saito
    Pages 51-64
  6. Xiaolei Su, Jonathon A. Ditlev, Michael K. Rosen, Ronald D. Vale
    Pages 65-76
  7. Enrique Calleja, Balbino Alarcón, Clara L. Oeste
    Pages 89-99
  8. Jérôme Bouchet, Iratxe del Río-Iñiguez, Andrés Alcover
    Pages 129-142
  9. Laura Patrussi, Cosima T. Baldari
    Pages 143-155
  10. Anastasios Siokis, Philippe A. Robert, Michael Meyer-Hermann
    Pages 171-182
  11. Valarie A. Barr, Jason Yi, Lawrence E. Samelson
    Pages 183-206
  12. Lining Ju, Yunfeng Chen, Muaz Nik Rushdi, Wei Chen, Cheng Zhu
    Pages 231-258
  13. Haogang Cai, David Depoil, James Muller, Michael P. Sheetz, Michael L. Dustin, Shalom J. Wind
    Pages 307-331
  14. Weiyang Jin, Charles T. Black, Lance C. Kam, Morgan Huse
    Pages 333-346

About this book


This volume provides all the essential protocols that are currently used to study the immune synapse. Chapters in The Immune Synapse: Methods and Protocols cover methods for the study of the dynamics of immune synapse assembly, traffic at the immune synapse, new high resolution imaging, biophysical and computational methods for the study of the immune synapse, effector immune synapses, B cell, NK and mast cell immune synapses, and immune interactions in vivo.Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, The Immune Synapse: Methods and Protocols will be of interest to immunologists and, at a more general level, to cell biologists, biophysicists and computational biologists.


T-cell immune synapse vesicular traffic B cell mast cell immune synapses immune interactions

Editors and affiliations

  • Cosima T. Baldari
    • 1
  • Michael L. Dustin
    • 2
  1. 1.Department of Life sciencesUniversity of SienaSienaItaly
  2. 2.University of Oxford, Kennedy Institute of RheumatologyHeadington, OxfordUnited Kingdom

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media LLC 2017
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-6879-4
  • Online ISBN 978-1-4939-6881-7
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book