Abstract
The diagnostic and prognostic value of microRNAs (miRNAs) in diseases becomes promising. Owing to fast response and high sensitivity, silicon nanowire (SiNW) biosensor has been considered a potential tool for miRNAs detection. Here, we describe a booming method to detect miRNAs with poly-silicon nanowire biosensors. Standard and real miRNA samples are applied in this study. The results show a limitation of 1 fM in the detection of standard miRNA sample with our poly-nanowire devices. Meanwhile, one-base mismatched sequence could be distinguished. Furthermore, these poly-SiNW arrays can detect snRNA U6 in total RNA samples extracted from HepG2 cells with a detection limitation of 0.2 μg/mL.
*Joint senior authors.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Bartel DP (2004) MicroRNAs: genomics, biogenesis, mechanism, and function. Cell 116:281–297
Tay Y, Zhang J, Thomson AM, Lim B, Rigoutsos I (2008) MicroRNAs to Nanog, Oct4 and Sox2 coding regions modulate embryonic stem cell differentiation. Nature 455:1124–1128
Guo D, Li Q, Lv Q, Wei Q, Cao S et al (2014) MiR-27a targets sFRP1 in hFOB cells to regulate proliferation, apoptosis and differentiation. PLoS One 9:e91354
Chen X, Wang X, Ruan A, Han W, Zhao Y et al (2014) miR-141 Is a Key Regulator of Renal Cell Carcinoma Proliferation and Metastasis by Controlling EphA2 Expression. Clin Cancer Res 20:2617–2630
Esquela-Kerscher A, Slack FJ (2006) Oncomirs - microRNAs with a role in cancer. Nat Rev Cancer 6:259–269
Lusis AJ (2000) Atherosclerosis. Nature 407:233–241
Brase JC, Wuttig D, Kuner R, Sultmann H (2010) Serum microRNAs as non-invasive biomarkers for cancer. Mol Cancer 9:306
Cortez MA, Bueso-Ramos C, Ferdin J, Lopez-Berestein G, Sood AK et al (2011) MicroRNAs in body fluids--the mix of hormones and biomarkers. Nat Rev Clin Oncol 8:467–477
de Planell-Saguer M, Rodicio MC (2013) Detection methods for microRNAs in clinic practice. Clin Biochem 46:869–878
Zhang GJ, Chua JH, Chee RE, Agarwal A, Wong SM (2009) Label-free direct detection of MiRNAs with silicon nanowire biosensors. Biosens Bioelectron 24:2504–2508
Dorvel BR, Reddy B Jr, Go J, Duarte Guevara C, Salm E et al (2012) Silicon nanowires with high-k hafnium oxide dielectrics for sensitive detection of small nucleic acid oligomers. ACS Nano 6:6150–6164
Acknowledgment
The analysis was supported by National Natural Science Foundation of China (NSFC 30800401 and 81201604).
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2017 Springer Science+Business Media LLC
About this protocol
Cite this protocol
He, J., Zhu, J., Jiang, B., Zhao, Y. (2017). Label-Free Direct Detection of MiRNAs with Poly-Silicon Nanowire Biosensors. In: Dalmay, T. (eds) MicroRNA Detection and Target Identification. Methods in Molecular Biology, vol 1580. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6866-4_20
Download citation
DOI: https://doi.org/10.1007/978-1-4939-6866-4_20
Published:
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-6864-0
Online ISBN: 978-1-4939-6866-4
eBook Packages: Springer Protocols