MicroRNA Detection and Target Identification

Methods and Protocols

  • Tamas Dalmay

Part of the Methods in Molecular Biology book series (MIMB, volume 1580)

Table of contents

  1. Front Matter
    Pages i-xii
  2. C. Jake Harris, David C. Baulcombe, Attila Molnar
    Pages 1-6
  3. Thorarinn Blondal, Maurizia Rossana Brunetto, Daniela Cavallone, Martin Mikkelsen, Michael Thorsen, Yuan Mang et al.
    Pages 21-44
  4. Katherine E. Richards, David B. Go, Reginald Hill
    Pages 59-70
  5. Maria Chiara Giuffrida, Roberta D’Agata, Giuseppe Spoto
    Pages 71-78
  6. Ludek Zavesky, Eva Jandakova, Radovan Turyna, Daniela Duskova, Lucie Langmeierova, Vit Weinberger et al.
    Pages 99-106
  7. Judith Hauptmann, Gunter Meister
    Pages 107-116
  8. Zixing Wang, Yin Liu
    Pages 117-126
  9. Raheleh Amirkhah, Hojjat Naderi Meshkin, Ali Farazmand, John E. J. Rasko, Ulf Schmitz
    Pages 127-147
  10. Cristina Gómez-Martín, Ricardo Lebrón, Antonio Rueda, José L. Oliver, Michael Hackenberg
    Pages 149-174
  11. Irina Mohorianu, Matthew Benedict Stocks, Christopher Steven Applegate, Leighton Folkes, Vincent Moulton
    Pages 193-224
  12. Eduardo Andrés-León, Gonzalo Gómez-López, David G. Pisano
    Pages 225-237
  13. Cristina Borzi, Linda Calzolari, Davide Conte, Gabriella Sozzi, Orazio Fortunato
    Pages 239-247
  14. Tanyu Wang, Gangli Wang, Didier Merlin, Emilie Viennois
    Pages 249-263
  15. Emma Bell, Hannah L. Watson, Shivani Bailey, Matthew J. Murray, Nicholas Coleman
    Pages 265-279
  16. Francesco Russo, Kirstine Belling, Anders Boeck Jensen, Flavia Scoyni, Søren Brunak, Marco Pellegrini
    Pages 281-295

About this book

Introduction

This volume is a collection of miRNA detection and target identification protocols, detailing new developments in the traditional detection approaches such as northern blot, quantitative real-time PCR, array, next generation sequencing, and in situ hybridization. The chapters in MicroRNA Detection and Target Identification: Methods and Protocols  guide readers through novel approaches such as nanotechnology, microfluidics, based detection methods, analysis of serum and urinary, miRNAs as biomarkers, target identification and experimental approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Authoritative and practical, MicroRNA Detection and Target Identification: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Keywords

array microfluidics next generation sequencing quantitative real-time PCR hybridization

Editors and affiliations

  • Tamas Dalmay
    • 1
  1. 1.School of Biological SciencesUniversity of East AngliaNorwichUnited Kingdom

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4939-6866-4
  • Copyright Information Springer Science+Business Media LLC 2017
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-6864-0
  • Online ISBN 978-1-4939-6866-4
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book