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Optimization of Satellite Cell Culture Through Biomaterials

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Muscle Stem Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1556))

Abstract

Hydrogels, a type of biomaterial, are an invaluable part of biomedical research as they are highly hydrated and properties such as elasticity, porosity, and ligand density can be tuned to desired values. Recently, culture substrate stiffness was found to be an important regulator of muscle stem cell self-renewal. Polyethylene glycol (PEG), a synthetic polymer, can be fabricated into hydrogels that match the softness of skeletal muscle tissue, thereby providing a culture surface that is optimal for maintaining muscle stem cell self-renewal potential ex vivo. In this Chapter, we describe a method to produce flat PEG hydrogels across a range of stiffnesses, including a formulation that matches the bulk stiffness of healthy skeletal muscle (12 kPa), while maintaining a constant ligand density. Since PEG is inert to protein adsorption, the steps required to surface functionalize the hydrogel with an adhesive interface (e.g., laminin) are also described.

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References

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Acknowledgment

The Natural Sciences and Engineering Research Council and the Canadian Institutes of Health Research supported this work.

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Authors and Affiliations

  1. Institute of Biomaterials and Biomedical Engineering, University of Toronto, 164 College Street Rm. 407, Toronto, M5S 3G9, Canada

    Sadegh Davoudi & Penney M. Gilbert

  2. Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, Canada

    Sadegh Davoudi & Penney M. Gilbert

Authors
  1. Sadegh Davoudi
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  2. Penney M. Gilbert
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Corresponding author

Correspondence to Penney M. Gilbert .

Editor information

Editors and Affiliations

  1. Cell Biology Group, Department of Experimental and Health Sciences, University Pompeu Fabra (UPF), Barcelona, Spain

    Eusebio Perdiguero

  2. Division of Biological Sciences and Christopher S. Bond Life Sciences Center, University of Missouri, Columbia, MO, USA

    DDW Cornelison

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Davoudi, S., Gilbert, P.M. (2017). Optimization of Satellite Cell Culture Through Biomaterials. In: Perdiguero, E., Cornelison, D. (eds) Muscle Stem Cells. Methods in Molecular Biology, vol 1556. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-6771-1_18

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  • DOI: https://doi.org/10.1007/978-1-4939-6771-1_18

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-6769-8

  • Online ISBN: 978-1-4939-6771-1

  • eBook Packages: Springer Protocols

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