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Assessment of Energy Metabolic Changes in Adipose Tissue-Derived Stem Cells

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Adult Stem Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1553))

Abstract

Adipose tissue-derived stem cells (ADSC) are promising candidates for therapeutic applications in cardiovascular regenerative medicine. By definition, the phenotype ADSCs, e.g., the ubiquitous secretion of growth factors, cytokines, and extracellular matrix components is not met in vivo, which renders ADSC a culture “artefact.” The medium constituents therefore impact the efficacy of ADSC. Little attention has been paid to the energy source in medium, i.e., glucose, which feeds the cell’s power plants: mitochondria. The role of mitochondria in stem cell biology goes beyond their function in ATP synthesis, because it includes cell signaling, reactive oxygen species (ROS) production, regulation of apoptosis, and aging. Appropriate application of ADSC for stem cells therapy of cardiovascular disease warrants knowledge of their mitochondrial phenotype and function. We discuss several methodologies for assessing ADSC mitochondrial function and structural changes under environmental cues, in particular, increased ROS caused by hyperglycemia.

The original version of this chapter was revised. The erratum to this chapter is available at: DOI 10.1007/978-1-4939-6756-8_27

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Acknowledgments

This project has received funding from the Marie Curie International Research Staff Exchange Scheme with the 7th European Community Framework Program under grant agreement No. 295185 - EULAMDIMA.

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Authors and Affiliations

  1. Cardiovascular Regenerative Medicine Research Group of the Department of Pathology and Medical Biology, University of Groningen, University Medical Center Groningen, Hanzeplein 1 – EA11, 9713 GZ, Groningen, The Netherlands

    Ghazaleh Hajmousa & Martin C. Harmsen

Authors
  1. Ghazaleh Hajmousa
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  2. Martin C. Harmsen
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Corresponding author

Correspondence to Martin C. Harmsen .

Editor information

Editors and Affiliations

  1. Center for Regenerative Medicine, Università di Roma Tor Vergata, Rome, Italy

    Paolo Di Nardo

  2. Institute of Cardiovascular Sciences St-Boniface Hospital Albrechtsen Research Centre Regenerative Medicine Program College of Medicine Faculty of Health Sciences, University of Manitoba, Winnipeg, Manitoba, Canada

    Sanjiv Dhingra

  3. Division of Metabolic and Cardiovascular Sciences Burnett School of Biomedical Sciences, University of Central Florida, Orlando, Florida, USA

    Dinender K. Singla

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Hajmousa, G., Harmsen, M.C. (2017). Assessment of Energy Metabolic Changes in Adipose Tissue-Derived Stem Cells. In: Di Nardo, P., Dhingra, S., Singla, D. (eds) Adult Stem Cells. Methods in Molecular Biology, vol 1553. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6756-8_5

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  • DOI: https://doi.org/10.1007/978-1-4939-6756-8_5

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6754-4

  • Online ISBN: 978-1-4939-6756-8

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