Abstract
Sequencing the entire genome of single sperm cells can provide valuable information of the distribution of meiotic recombination events in eukaryotic genomes. Here, we provide a description of the experimental work flow for isolating single sperm cells from the microcrustacean Daphnia pulex using fluorescence-activated cell sorting. Moreover, we describe the application of a whole-genome amplification technique (i.e., Multiple Annealing and Looping Based Amplification Cycles method) to single sperm of Daphnia to generate enough DNA for library preparation of next-generation sequencing.
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Acknowledgments
We would like to thank Drs. L. J. Bright, T. Doak, K. Spitze, M. Ackerman, and M. Lynch for their technical help and stimulating discussion. Dr. Hongan Long prepared the drawing of Fig. 1.
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Xu, S., Young, K. (2017). Whole-Genome Haplotyping of Single Sperm of Daphnia pulex (Crustacea, Anomopoda). In: Tiemann-Boege, I., Betancourt, A. (eds) Haplotyping. Methods in Molecular Biology, vol 1551. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6750-6_8
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DOI: https://doi.org/10.1007/978-1-4939-6750-6_8
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