Abstract
Hepatitis B virus (HBV) is a hepatotropic virus. Its infection can cause liver injury and regeneration, and its replication is affected by the gender. Transgenic mice that carry a 1.3-mer overlength HBV DNA genome productively replicate HBV in hepatocytes and have been very useful for studying the replication and pathogenesis of HBV in vivo. By using this mouse model, the relationship between HBV and liver injury and regeneration as well as the effect of the gender on HBV gene expression and replication has been studied. In this chapter, we describe the surgical procedures of partial hepatectomy and castration and provide examples to demonstrate how these surgical procedures may be used to study the effect of HBV on liver regeneration and the effect of androgen on HBV replication.
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References
Yu MW, Yang YC, Yang SY, Cheng SW, Liaw YF, Lin SM, Chen CJ (2001) Hormonal markers and hepatitis B virus-related hepatocellular carcinoma risk: a nested case-control study among men. J Natl Cancer Inst 93(21):1644–1651
Fausto N, Campbell JS, Riehle KJ (2006) Liver regeneration. Hepatology 43(2 Suppl 1):S45–S53
Chisari FV (1995) Hepatitis B virus transgenic mice: insights into the virus and the disease. Hepatology 22(4 Pt 1):1316–1325
Chisari FV (1996) Hepatitis B virus transgenic mice: models of viral immunobiology and pathogenesis. Curr Top Microbiol Immunol 206:149–173
Tian Y, Kuo CF, Chen WL, Ou JH (2012) Enhancement of hepatitis B virus replication by androgen and its receptor in mice. J Virol 86(4):1904–1910
Higgins GMA, Anderson RM (1931) Experimental pathology of the liver: I. Restoration of the liver of the white rat following partial surgical removal. Arch Pathol 12:186–202
Mitchell C, Willenbring H (2008) A reproducible and well-tolerated method for 2/3 partial hepatectomy in mice. Nat Protoc 3(7):1167–1170
Nikfarjam M, Malcontenti-Wilson C, Fanartzis M, Daruwalla J, Christophi C (2004) A model of partial hepatectomy in mice. J Invest Surg 17(5):291–294
Inderbitzin D, Studer P, Sidler D, Beldi G, Djonov V, Keogh A, Candinas D (2006) Regenerative capacity of individual liver lobes in the microsurgical mouse model. Microsurgery 26(6):465–469
Greene AK, Puder M (2003) Partial hepatectomy in the mouse: technique and perioperative management. J Invest Surg 16(2):99–102
Hori T, Ohashi N, Chen F, Baine AM, Gardner LB, Jermanus S, Nguyen JH (2011) Simple and sure methodology for massive hepatectomy in the mouse. Ann Gastroenterol 24(4):307–318
Hori T, Ohashi N, Chen F, Baine AM, Gardner LB, Hata T, Uemoto S, Nguyen JH (2012) Simple and reproducible hepatectomy in the mouse using the clip technique. World J Gastroenterol 18(22):2767–2774
Dervan PA, Magee HM, Buckley C, Carney DN (1992) Proliferating cell nuclear antigen counts in formalin-fixed paraffin-embedded tissue correlate with Ki-67 in fresh tissue. Am J Clin Pathol 97(5 Suppl 1):S21–S28
Mancini R, Marucci L, Benedetti A, Jezequel AM, Orlandi F (1994) Immunohistochemical analysis of S-phase cells in normal human and rat liver by PC10 monoclonal antibody. Liver 14(2):57–64
Hall PA, Levison DA, Woods AL, Yu CC, Kellock DB, Watkins JA, Barnes DM, Gillett CE, Camplejohn R, Dover R et al (1990) Proliferating cell nuclear antigen (PCNA) immunolocalization in paraffin sections: an index of cell proliferation with evidence of deregulated expression in some neoplasms. J Pathol 162(4):285–294
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Tian, Y., Ou, Jh.J. (2017). Partial Hepatectomy and Castration of HBV Transgenic Mice. In: Guo, H., Cuconati, A. (eds) Hepatitis B Virus. Methods in Molecular Biology, vol 1540. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6700-1_22
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DOI: https://doi.org/10.1007/978-1-4939-6700-1_22
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