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Hepatitis B Virus

Methods and Protocols

  • Haitao Guo
  • Andrea Cuconati

Part of the Methods in Molecular Biology book series (MIMB, volume 1540)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Yinyan Sun, Yonghe Qi, Bo Peng, Wenhui Li
    Pages 1-14
  3. Quentin Osseman, Michael Kann
    Pages 37-51
  4. Ching-Chun Yang, Hung-Cheng Li, Chiaho Shih
    Pages 53-58
  5. Yuchen Xia, Daniela Stadler, Chunkyu Ko, Ulrike Protzer
    Pages 59-72
  6. Kristie Bloom, Abdullah Ely, Patrick Arbuthnot
    Pages 85-95
  7. Daniel N. Clark, Scott A. Jones, Jianming Hu
    Pages 157-177
  8. Cally D. Goddard, Lale Bildrici-Ertekin, Xiaohe Wang, Andrea Cuconati
    Pages 203-209
  9. Yanli Qin, Yong-Xiang Wang, Jiming Zhang, Jisu Li, Shuping Tong
    Pages 219-226
  10. Li-Ling Wu, Hurng-Yi Wang, Pei-Jer Chen
    Pages 227-235
  11. Tracy D. Reynolds, Safiehkhatoon Moshkani, Michael D. Robek
    Pages 237-247
  12. Jia Liu, Xuan Huang, Melanie Werner, Ruth Broering, Dongliang Yang, Mengji Lu
    Pages 249-258
  13. Yongjun Tian, Jing-hsiung James Ou
    Pages 259-266
  14. Marta G. Murreddu, Manasa Suresh, Severin O. Gudima, Stephan Menne
    Pages 277-294
  15. Back Matter
    Pages 295-297

About this book

Introduction

This volume serves as a reference for the dissemination of advances made in the study of Hepatitis B Virus (HBV). Hepatitis B Virus: Methods and Protocols details protocols and techniques ranging from cell culture studies to in vivo and clinical immunology. The chapters in this book discuss treatments of in vitro infection systems, analysis and quantification of cccDNA and its mutations; in vitro polymerase activity assays; cellular trafficking of core proteins; intracellular calcium metabolism; detection, cloning, and sequencing of HBV markers; and new strategies aimed at exploiting new mechanisms for drug discovery. The book also covers classical methods for resolution of extracellular viral particles by native gel electrophoresis, and methods for detecting HBV antigens in drug discovery. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Hepatitis B Virus: Methods and Protocols is a valuable tool for researchers to use toward their advanced studies in HBV.

 

Keywords

DNA and RNA viruses covalently closed circular (ccc) DNA genome immunotolerance in vitro polymerase activity assays intracellular calcium metabolism gel electrophoresis drug discovery

Editors and affiliations

  • Haitao Guo
    • 1
  • Andrea Cuconati
    • 2
  1. 1.Department of Microbiology and ImmunologyIndiana University School of MedicineIndianapolisUSA
  2. 2.Arbutus Biopharma, Inc.DoylestownUSA

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4939-6700-1
  • Copyright Information Springer Science+Business Media LLC 2017
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-6698-1
  • Online ISBN 978-1-4939-6700-1
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site