Editors:
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 1540)
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Table of contents (24 protocols)
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Front Matter
About this book
This volume serves as a reference for the dissemination of advances made in the study of Hepatitis B Virus (HBV). Hepatitis B Virus: Methods and Protocols details protocols and techniques ranging from cell culture studies to in vivo and clinical immunology. The chapters in this book discuss treatments of in vitro infection systems, analysis and quantification of cccDNA and its mutations; in vitro polymerase activity assays; cellular trafficking of core proteins; intracellular calcium metabolism; detection, cloning, and sequencing of HBV markers; and new strategies aimed at exploiting new mechanisms for drug discovery. The book also covers classical methods for resolution of extracellular viral particles by native gel electrophoresis, and methods for detecting HBV antigens in drug discovery. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and comprehensive, Hepatitis B Virus: Methods and Protocols is a valuable tool for researchers to use toward their advanced studies in HBV.
Editors and Affiliations
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Department of Microbiology and Immunology, Indiana University School of Medicine, Indianapolis, USA
Haitao Guo
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Arbutus Biopharma, Inc., Doylestown, USA
Andrea Cuconati
Bibliographic Information
Book Title: Hepatitis B Virus
Book Subtitle: Methods and Protocols
Editors: Haitao Guo, Andrea Cuconati
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-6700-1
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media LLC 2017
Hardcover ISBN: 978-1-4939-6698-1Published: 15 December 2016
Softcover ISBN: 978-1-4939-8277-6Published: 08 June 2018
eBook ISBN: 978-1-4939-6700-1Published: 13 December 2016
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XII, 297
Number of Illustrations: 26 b/w illustrations, 31 illustrations in colour
Topics: Infectious Diseases