Abstract
Bicellar mixtures have been used as alignable membrane substrates for the structural characterization of membrane-associated proteins. Most recently, it has been shown that bicelles can serve as nanocarriers to effectively deliver hydrophobic molecules to cancer cells with a 3- to 10-fold enhancement compared to that of chemically identical liposomes. In this chapter, a detailed preparation protocol, common structural characterization methods, the structural stability and the cellular uptake of bicellar nanodisks are discussed.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Sanders CR, Landis GC (1995) Reconstitution of membrane proteins into lipid-rich bilayered mixed micelles for NMR studies. Biochemistry 34(12):4030–4040
Diller A, Loudet C, Aussenac F et al (2009) Bicelles: a natural ‘molecular goniometer’ for structural, dynamical and topological studies of molecules in membranes. Biochimie 91(6):744–751
Naito A (2009) Structure elucidation of membrane-associated peptides and proteins in oriented bilayers by solid-state NMR spectroscopy. Solid State Nucl. Magn Reson 36(2):67–76
Poget SF, Girvin ME (2007) Solution NMR of membrane proteins in bilayer mimics: small is beautiful, but sometimes bigger is better. Biochim Biophys Acta 1768(12):3098–3106
Prosser RS, Evanics F, Kitevski JL et al (2006) Current applications of bicelles in NMR studies of membrane-associated amphiphiles and proteins. Biochemistry 45(28):8453–8465
Johansson LC, Wohri AB, Katona G et al (2009) Membrane protein crystallization from lipidic phases. Curr Opin Struct Biol 19(4):372–378
Kimble-Hill AC (2013) A review of factors affecting the success of membrane protein crystallization using bicelles. Front Biol 8(3):261–272
Poulos S, Morgan JL, Zimmer J et al (2015) Bicelles coming of age: an empirical approach to bicelle crystallization. Methods Enzymol 557:393–416
Mills JO, Holland LA (2004) Membrane-mediated capillary electrophoresis: interaction of cationic peptides with bicelles. Electrophoresis 25(9):1237–1242
Barbosa-Barros L, Barba C, Rodriguez G et al (2009) Lipid nanostructures: self-assembly and effect on skin properties. Mol Pharm 6(4):1237–1245
Rodriguez G, Barbosa-Barros L, Rubio L et al (2009) Conformational changes in stratum corneum lipids by effect of bicellar systems. Langmuir 25(18):10595–10603
Rodriguez G, Barbosa-Barros L, Rubio L et al (2015) Bicelles: New Lipid Nanosystems for Dermatological Applications. J Biomed Nanotechnol 11(2):282–290
Aresh, W Liu, Y., Sine, J. et al.The Morphology of Self-Assembled Lipid-Based Nanoparticles Affects Their Uptake by Cancer Cells. Journal of Biomedical Nanotechnology (in press).
Katsaras J, Harroun TA, Pencer J et al (2005) "Bicellar" lipid mixtures as used in biochemical and biophysical studies. Naturwissenschaften 92(8):355–366
Andersson A, Maler L (2006) Size and shape of fast-tumbling bicelles as determined by translational diffusion. Langmuir 22(6):2447–2449
Beaugrand M, Arnold AA, Henin J et al (2014) Lipid concentration and molar ratio boundaries for the use of isotropic bicelles. Langmuir 30(21):6162–6170
Lind J, Nordin J, Maler L (2008) Lipid dynamics in fast-tumbling bicelles with varying bilayer thickness: effect of model transmembrane peptides. Biochim Biophys Acta 1778(11):2526–2534
Ye W, Lind J, Eriksson J et al (2014) Characterization of the morphology of fast-tumbling bicelles with varying composition. Langmuir 30(19):5488–5496
Nieh M-P, Dolinar P, Kučerka N et al (2011) Formation of kinetically trapped nanoscopic unilamellar vesicles from metastable nanodiscs. Langmuir 27(23):14308–14316
Soong R, Nieh M-P, Nicholson E et al (2010) Bicellar mixtures containing pluronic F68: morphology and lateral diffusion from combined SANS and PFG NMR studies. Langmuir 26(4):2630–2638
Yang P-W, Liu T-L, Hu Y et al (2012) Small-Angle X-ray Scattering studies on the structure of mixed DPPC/diC7PC micelles in aqueous solutions. Chinese Journal of Physics 50(2):349–356
Li M, Morales HH, Katsaras J et al (2013) Morphological characterization of DMPC/CHAPSO bicellar mixtures: a combined SANS and NMR study. Langmuir 29(51):15943–15957
Nieh M-P, Raghunathan VA, Kline SR et al (2005) Spontaneously formed unilamellar vesicles with path-dependent size distribution. Langmuir 21(15):6656–6661
Nieh M-P, Raghunathan VA, Pabst G et al (2011) Temperature driven annealing of perforations in bicellar model membranes. Langmuir 27(8):4838–4847
Mahabir S, Wan W, Katsaras J et al (2010) Effects of charge density and thermal history on the morphologies of spontaneously formed unilamellar vesicles. J Phys Chem B 114(17):5729–5735
Hu A, Fan T-H, Katsaras J et al (2014) Lipid-based nanodiscs as models for studying mesoscale coalescence--a transport limited case. Soft Matter 10(28):5055–5060
Kline SR (2006) Reduction and analysis of SANS and USANS data using IGOR Pro. J Appl Crystallogr 39(6):895–900
Liu Y, Li M, Yang Y et al (2014) The effects of temperature, salinity, concentration and PEGylated lipid on the spontaneous nanostructures of bicellar mixtures. Biochim Biophys Acta 1838(7):1871–1880
Acknowledgments
The authors would like to acknowledge the funding support from NSF-CMMI 1131587 and CBET 1433903.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2017 Springer Science+Business Media New York
About this protocol
Cite this protocol
Liu, Y., Xia, Y., Rad, A.T., Aresh, W., Nieh, MP. (2017). Stable Discoidal Bicelles: A Platform of Lipid Nanocarriers for Cellular Delivery. In: D'Souza, G. (eds) Liposomes. Methods in Molecular Biology, vol 1522. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6591-5_22
Download citation
DOI: https://doi.org/10.1007/978-1-4939-6591-5_22
Published:
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-6589-2
Online ISBN: 978-1-4939-6591-5
eBook Packages: Springer Protocols