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Liposomes

Methods and Protocols

  • Book
  • © 2017

Overview

Editors:
  1. Gerard G.M. D'Souza

    1. School of Pharmacy – Boston, MCPHS University, Boston, USA

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1522)

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Table of contents (22 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Liposomes Came First: The Early History of Liposomology

    • Volkmar Weissig
    Pages 1-15

  3. Thin-Film Hydration Followed by Extrusion Method for Liposome Preparation

    • Hongwei Zhang
    Pages 17-22

  4. Preparation of DRV Liposomes

    • Sophia G. Antimisiaris
    Pages 23-47

  5. Method of Simultaneous Analysis of Liposome Components Using HPTLC/FID

    • Sophia Hatziantoniou, Costas Demetzos
    Pages 49-54

  6. Freeze-Fracture Electron Microscopy on Domains in Lipid Mono- and Bilayer on Nano-Resolution Scale

    • Brigitte Papahadjopoulos-Sternberg
    Pages 55-72

  7. Liposome Formulations of Hydrophobic Drugs

    • Reto A. Schwendener, Herbert Schott
    Pages 73-82

  8. A “Dock and Lock” Approach to Preparation of Targeted Liposomes

    • Marina V. Backer, Joseph M. Backer
    Pages 83-92

  9. Coupling of Ligands to the Liposome Surface by Click Chemistry

    • Maria Vittoria Spanedda, Marcella De Giorgi, Fatouma Saïd Hassane, Francis Schuber, Line Bourel-Bonnet, Benoît Frisch
    Pages 93-106

  10. Elastic Liposomes for Topical and Transdermal Drug Delivery

    • Heather A. E. Benson
    Pages 107-117

  11. Determination of the Subcellular Distribution of Liposomes Using Confocal Microscopy

    • Melani A. Solomon
    Pages 119-130

  12. Environmental Scanning Electron Microscope Imaging of Vesicle Systems

    • Yvonne Perrie, Habib Ali, Daniel J Kirby, Afzal U. R. Mohammed, Sarah E. McNeil, Anil Vangala
    Pages 131-143

  13. Liposome Biodistribution via Europium Complexes

    • Nathalie Mignet, Daniel Scherman
    Pages 145-154

  14. Techniques for Loading Technetium-99m and Rhenium-186/188 Radionuclides into Preformed Liposomes for Diagnostic Imaging and Radionuclide Therapy

    • Beth Goins, Ande Bao, William T. Phillips
    Pages 155-178

  15. Gadolinium-Loaded Polychelating Polymer-Containing Tumor-Targeted Liposomes

    • Suna Erdogan, Vladimir P. Torchilin
    Pages 179-192

  16. Serum-Stable, Long-Circulating, pH-Sensitive PEGylated Liposomes

    • Nicolas Bertrand, Pierre Simard, Jean-Christophe Leroux
    Pages 193-207

  17. Long-Circulating, pH-Sensitive Liposomes

    • Denitsa Momekova, Stanislav Rangelov, Nikolay Lambov
    Pages 209-226

  18. Anionic pH-Sensitive Lipoplexes

    • Nathalie Mignet, Daniel Scherman
    Pages 227-236

  19. Fluorometric Analysis of Individual Cationic Lipid–DNA Complexes

    • Edwin Pozharski
    Pages 237-243

  20. Preparation and Physical Characterization of DNA-Binding Cationic Liposomes

    • Vaibhav Saxena
    Pages 245-250
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Keywords

About this book

This second edition volume expands on the previous edition by discussing classic techniques, as well as new protocols that focus on the preparation of liposomes, lipid characterization, particle size and charge analysis, drug encapsulation, surface modification, stimuli response, and cellular interaction and biodistribution. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and practical, Liposomes: Methods and Protocols, Second Edition is a valuable resource for graduate students, post-doctoral researchers, and established investigators utilizing lipid-based systems in the fields of cell and molecular biology, drug delivery, and physical chemistry.

Editors and Affiliations

  • School of Pharmacy – Boston, MCPHS University, Boston, USA

    Gerard G.M. D'Souza

Bibliographic Information

  • Book Title: Liposomes

  • Book Subtitle: Methods and Protocols

  • Editors: Gerard G.M. D'Souza

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-6591-5

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2017

  • Hardcover ISBN: 978-1-4939-6589-2Published: 11 November 2016

  • Softcover ISBN: 978-1-4939-8244-8Published: 07 July 2018

  • eBook ISBN: 978-1-4939-6591-5Published: 09 November 2016

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XI, 285

  • Number of Illustrations: 46 b/w illustrations, 13 illustrations in colour

  • Topics: Pharmacology/Toxicology

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