Abstract
The capacity of mammary myoepithelial cells to contract in response to suckling stimuli is essential for lactation. We describe here a protocol for studying the contractile activity of myoepithelial cells in vitro. This protocol includes the establishment of stable myoepithelial cell lines from mouse mammary glands and quantitative evaluation of the contraction and subsequent relaxation of cultured myoepithelial cells in response to oxytocin. It can be used for analyses of mouse mutants with gene deletions or overexpression altering myoepithelial cell function.
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Acknowledgments
The work in the team of M.A.G. was supported by La Ligue Nationale Contre le Cancer (Equipe Labellisée 2013) and grants from Agence Nationale de la Recherche ANR-08-BLAN-0078-01 and ANR-13-BSV2-0001. M.A.G. is Directeur de Recherche and K.R. is Chargé de Recherche at the Institut National de la Santé et de la Recherche Médicale (INSERM).
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Cagnet, S., Glukhova, M.A., Raymond, K. (2017). Contractility Assay for Established Myoepithelial Cell Lines. In: Martin, F., Stein, T., Howlin, J. (eds) Mammary Gland Development. Methods in Molecular Biology, vol 1501. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6475-8_8
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DOI: https://doi.org/10.1007/978-1-4939-6475-8_8
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-6473-4
Online ISBN: 978-1-4939-6475-8
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