Abstract
The first signaling peptide discovered and purified was insulin in 1921. However, it was not until 1991 that the first peptide signal, systemin, was discovered in plants. Since the discovery of systemin, peptides have emerged as a potent and diverse class of signaling molecules in plant systems. Peptides consist of small amino acid sequences, which often act as ligands of receptor kinases. However, not all peptides are created equal, and signaling peptides are grouped into several subgroups dependent on the type of post-translational processing they undergo. Here, we focus on the application of synthetic, post-translationally modified peptides (PTMPs) to plant systems, describing several methods appropriate for the use of peptides in Arabidopsis thaliana and crop models.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Murphy E, Smith S, De Smet I (2012) Small signaling peptides in Arabidopsis development: how cells communicate over a short distance. Plant Cell 24(8):3198–3217
Czyzewicz N, Yue K, Beeckman T et al (2013) Message in a bottle: small signalling peptide outputs during growth and development. J Exp Bot 64(17):5281–5296
Tavormina P, De Coninck B, Nikonorova N et al (2015) The plant peptidome: an expanding repertoire of structural features and biological functions. Plant Cell 27(8):2095–2118
Petraglia F, Florio P, Nappi C et al (2013) Peptide signaling in human placenta and membranes: autocrine, paracrine, and endocrine mechanisms. Endocr Rev 17(2):156–186
Reid D, Li D, Ferguson B et al (2013) Structure–function analysis of the GmRIC1 signal peptide and CLE domain required for nodulation control in soybean. J Exp Bot 64(6):1575–1585
Shinohara H, Matsubayashi Y (2013) Chemical synthesis of Arabidopsis CLV3 glycopeptide reveals the impact of hydroxyproline arabinosylation on peptide conformation and activity. Plant Cell Physiol 54(3):369–374
Czyzewicz N, Shi CL, Vu LD et al (2015) Modulation of Arabidopsis and monocot root architecture by CLAVATA3/EMBRYO SURROUNDING REGION 26 peptide. J Exp Bot 66(17):5229–5243
Qi Z, Verma R, Gehring C et al (2010) Ca2+ signaling by plant Arabidopsis thaliana Pep peptides depends on AtPepR1, a receptor with guanylyl cyclase activity, and cGMP-activated Ca2+ channels. Proc Natl Acad Sci U S A 107(49):21193–21198
Butenko M, Wildhagen M, Albert M et al (2014) Tools and strategies to match peptide-ligand receptor pairs. Plant Cell 26(5):1838–1847
Czyzewicz N, Wildhagen M, Cattaneo P et al (2015) Antagonistic peptide technology for functional dissection of CLE peptides revisited. J Exp Bot 66(17):5367–5374
Xu T, Ren S, Song X et al (2015) CLE19 expressed in the embryo regulates both cotyledon establishment and endosperm development in Arabidopsis. J Exp Bot 66(17):5217–5227
Mueller K, Chinchilla D, Albert M et al (2012) Contamination risks in work with synthetic peptides: flg22 as an example of a pirate in commercial peptide preparations. Plant Cell 24(8):3193–3197
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2017 Springer Science+Business Media New York
About this protocol
Cite this protocol
Czyzewicz, N., Stes, E., De Smet, I. (2017). Tips and Tricks for Exogenous Application of Synthetic Post-translationally Modified Peptides to Plants. In: Kleine-Vehn, J., Sauer, M. (eds) Plant Hormones. Methods in Molecular Biology, vol 1497. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6469-7_3
Download citation
DOI: https://doi.org/10.1007/978-1-4939-6469-7_3
Published:
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-6467-3
Online ISBN: 978-1-4939-6469-7
eBook Packages: Springer Protocols