Book Volume 1497 2017

Plant Hormones

Methods and Protocols

Editors:

ISBN: 978-1-4939-6467-3 (Print) 978-1-4939-6469-7 (Online)

Table of contents (22 protocols)

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  1. Front Matter

    Pages i-xi

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    Protocol

    Pages 1-8

    Real-Time Analysis of the Apical Hook Development

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    Protocol

    Pages 9-18

    Grafting with Arabidopsis thaliana

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    Protocol

    Pages 19-28

    Tips and Tricks for Exogenous Application of Synthetic Post-translationally Modified Peptides to Plants

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    Protocol

    Pages 29-36

    Assaying Germination and Seedling Responses of Arabidopsis to Karrikins

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    Protocol

    Pages 37-45

    Low-Cost Microprocessor-Controlled Rotating Stage for Medium-Throughput Time-Lapse Plant Phenotyping

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    Protocol

    Pages 47-55

    Genome-Wide Association Mapping of Root Traits in the Context of Plant Hormone Research

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    Protocol

    Pages 57-72

    High-Throughput Scoring of Seed Germination

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    Protocol

    Pages 73-80

    Histochemical Staining of β-Glucuronidase and Its Spatial Quantification

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    Protocol

    Pages 81-90

    Imaging TCSn::GFP, a Synthetic Cytokinin Reporter, in Arabidopsis thaliana

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    Protocol

    Pages 91-97

    Highlighting Gibberellins Accumulation Sites in Arabidopsis thaliana Root Using Fluorescently Labeled Gibberellins

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    Protocol

    Pages 99-123

    In Silico Methods for Cell Annotation, Quantification of Gene Expression, and Cell Geometry at Single-Cell Resolution Using 3DCellAtlas

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    Protocol

    Pages 125-133

    Analyzing Cell Wall Elasticity After Hormone Treatment: An Example Using Tobacco BY-2 Cells and Auxin

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    Protocol

    Pages 135-146

    FRET-FLIM for Visualizing and Quantifying Protein Interactions in Live Plant Cells

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    Protocol

    Pages 147-158

    In Vivo Identification of Plant Protein Complexes Using IP-MS/MS

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    Protocol

    Pages 159-191

    Assaying Auxin Receptor Activity Using SPR Assays with F-Box Proteins and Aux/IAA Degrons

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    Protocol

    Pages 193-203

    Studying Transcription Factor Binding to Specific Genomic Loci by Chromatin Immunoprecipitation (ChIP)

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    Protocol

    Pages 205-220

    Hormone Receptor Glycosylation

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    Protocol

    Pages 221-229

    Highly Sensitive Salicylic Acid Quantification in Milligram Amounts of Plant Tissue

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    Protocol

    Pages 231-248

    High-Resolution Cell-Type Specific Analysis of Cytokinins in Sorted Root Cell Populations of Arabidopsis thaliana

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    Protocol

    Pages 249-258

    Hormone Profiling in Plant Tissues

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