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Use of Xenopus laevis Oocytes to Study Auxin Transport

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Plant Hormones

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1497))

Abstract

Xenopus laevis oocytes are an expression system that is particularly well suited for the characterization of membrane transporters. Oocytes possess only very little endogenous transport systems and therefore transporters can be studied with a high signal-to-noise ratio. This book chapter provides the basic methods to use Xenopus oocytes for the characterization of transporters by radiotracer experiments. While the methods described here were established to study auxin transport they can easily be adapted to study other hormone transporters and their substrates.

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Acknowledgement

This work was supported by DFG grant HA3469/6-1.

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Authors and Affiliations

  1. Cell Biology and Plant Biochemistry, University of Regensburg, Universitätsstraße 31, 93053, Regensburg, Germany

    Astrid Fastner, Birgit Absmanner & Ulrich Z. Hammes

Authors
  1. Astrid Fastner
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  2. Birgit Absmanner
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  3. Ulrich Z. Hammes
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Corresponding author

Correspondence to Ulrich Z. Hammes .

Editor information

Editors and Affiliations

  1. IAGZ, Universität für Bodenkultur Wien, Vienna, Wien, Austria

    Jürgen Kleine-Vehn

  2. Institut für Biochemie und Biologie, Universität Potsdam, Potsdam, Germany

    Michael Sauer

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Fastner, A., Absmanner, B., Hammes, U.Z. (2017). Use of Xenopus laevis Oocytes to Study Auxin Transport. In: Kleine-Vehn, J., Sauer, M. (eds) Plant Hormones. Methods in Molecular Biology, vol 1497. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6469-7_21

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  • DOI: https://doi.org/10.1007/978-1-4939-6469-7_21

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6467-3

  • Online ISBN: 978-1-4939-6469-7

  • eBook Packages: Springer Protocols

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