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Analysis of MicroRNA Function in Drosophila

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Drosophila

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1478))

Abstract

MicroRNAs are short noncoding, ~22-nucleotide RNAs that regulate protein abundance. The growth in our understanding of this class of RNAs has been rapid since their discovery just over a decade ago. We now appreciate that miRNAs are deeply embedded within the genetic networks that control basic features of metazoan cells including their identity, metabolism, and reproduction. The Drosophila melanogaster model system has made and will continue to make important contributions to this analysis. Intended as an introductory overview, here we review the current methods and resources available for functional analysis of fly miRNAs for those interested in performing this type of analysis.

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    Authors and Affiliations

    1. Department of Biology, Indiana University, Jordan Hall, 1001 East Third St., Bloomington, IN, 47405, USA

      Geetanjali Chawla, Arthur Luhur & Nicholas Sokol

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    1. Geetanjali Chawla
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    2. Arthur Luhur
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    3. Nicholas Sokol
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    Correspondence to Nicholas Sokol .

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    Editors and Affiliations

    1. Institute of Genetics, Technische Universität Dresden, Dresden, Germany

      Christian Dahmann

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    Chawla, G., Luhur, A., Sokol, N. (2016). Analysis of MicroRNA Function in Drosophila . In: Dahmann, C. (eds) Drosophila. Methods in Molecular Biology, vol 1478. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6371-3_4

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    • DOI: https://doi.org/10.1007/978-1-4939-6371-3_4

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