Advertisement

Drosophila

Methods and Protocols

  • Christian Dahmann

Part of the Methods in Molecular Biology book series (MIMB, volume 1478)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Steven J. Marygold, Madeline A. Crosby, Joshua L. Goodman, The FlyBase Consortium
    Pages 1-31
  3. Olena Riabinina, Christopher J. Potter
    Pages 53-78
  4. Geetanjali Chawla, Arthur Luhur, Nicholas Sokol
    Pages 79-94
  5. Maximilian Billmann, Michael Boutros
    Pages 95-116
  6. Aynur Kaya-Çopur, Frank Schnorrer
    Pages 117-143
  7. Fillip Port, Simon L. Bullock
    Pages 145-160
  8. Anne Pélissier-Monier, Bénédicte Sanson, Bruno Monier
    Pages 161-176
  9. Emmanuel Caussinus, Markus Affolter
    Pages 177-187
  10. Simon Restrepo, Jeremiah J. Zartman, Konrad Basler
    Pages 203-213
  11. Maureen Cetera, Lindsay Lewellyn, Sally Horne-Badovinac
    Pages 215-226
  12. Benoit Aigouy, Daiki Umetsu, Suzanne Eaton
    Pages 227-239
  13. Pruthvi C. Shivakumar, Pierre-François Lenne
    Pages 241-251
  14. Katrin Strassburger, Aurelio A. Teleman
    Pages 279-290
  15. Matthew D. W. Piper, Linda Partridge
    Pages 291-302
  16. Wendi S. Neckameyer, Parag Bhatt
    Pages 303-320
  17. Alain Debec, Timothy L. Megraw, Antoine Guichet
    Pages 333-351
  18. Back Matter
    Pages 353-355

About this book

Introduction

This second edition volume expands on the previous edition by presenting updated protocols for several of the techniques described in the first edition of Drosophila: Methods and Protocols, and current methods that cover recent breakthroughs in Drosophila research. The book begins with a description of FlyBase--a database of genes and genomes--followed by the presentation of systems for versatile gene expression in the fly. The first few chapters in this book detail gene knockdown and editing, including CRISPR-Cas9 and protein knockdown. The next few chapters are devoted to methods describing live imaging of different tissues and organs, followed by chapters on how to quantify image data and how to probe tissue mechanics by laser ablation. The next two chapters provide methods for analyzing transcription followed by protocols to study growth, metabolism, ageing, and behavior in Drosophila. This volume concludes with chapters on electrophysiological recordings and methods to establish cell lines. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Drosophila: Methods and Protocols, Second Edition is a valuable source of hands-on protocols and reviews for molecular, cell, and developmental biologists using Drosophila as model systems in their work.

Keywords

Drosophila melanogaster use of transposons to generate transgenic flies FlyBase gene expression gene and protein knockdown CRISPR-Cas9 live imaging electrophysiological recordings

Editors and affiliations

  • Christian Dahmann
    • 1
  1. 1.Institute of GeneticsTechnische Universität DresdenDresdenGermany

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4939-6371-3
  • Copyright Information Springer Science+Business Media New York 2016
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-6369-0
  • Online ISBN 978-1-4939-6371-3
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site