Abstract
Overlapping PCR is commonly used in many molecular applications that include stitching PCR fragments together, generating fluorescent transcriptional and translational fusions, inserting mutations, making deletions, and PCR cloning. Overlapping PCR is also used for genotyping and in detection experiments using techniques such as loop-mediated isothermal amplification (LAMP). STITCHER is a web tool providing a central resource for researchers conducting all types of overlapping assembly PCR experiments with an intuitive interface for automated primer design that’s fast, easy to use, and freely available online.
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Acknowledgements
Support for this work was provided by The George Washington University (GWU) Columbian College of Arts and Sciences, GWU Office of the Vice President for Research, and the GWU Department of Biological Sciences.
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O’Halloran, D.M. (2017). A Guide to Using STITCHER for Overlapping Assembly PCR Applications. In: Hughes, R. (eds) Synthetic DNA. Methods in Molecular Biology, vol 1472. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6343-0_1
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DOI: https://doi.org/10.1007/978-1-4939-6343-0_1
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