Book Volume 1472 2017

Synthetic DNA

Methods and Protocols

Editors:

ISBN: 978-1-4939-6341-6 (Print) 978-1-4939-6343-0 (Online)

Table of contents (18 protocols)

  1. Front Matter

    Pages i-xiii

  2. Computational Tools for Design and Assembly of Synthetic DNA

    1. Front Matter

      Pages 1-1

    2. No Access

      Protocol

      Pages 3-11

      A Guide to Using STITCHER for Overlapping Assembly PCR Applications

    3. No Access

      Protocol

      Pages 13-34

      Synthetic Gene Design Using Codon Optimization On-Line (COOL)

    4. No Access

      Protocol

      Pages 35-45

      Shuffle Optimizer: A Program to Optimize DNA Shuffling for Protein Engineering

  3. DNA Synthesis, Assembly, and Cloning

    1. Front Matter

      Pages 47-47

    2. No Access

      Protocol

      Pages 49-61

      Simple Cloning by Prolonged Overlap Extension-PCR with Application to the Preparation of Large-Size Random Gene Mutagenesis Library in Escherichia coli

    3. No Access

      Protocol

      Pages 63-78

      SpeedyGenes: Exploiting an Improved Gene Synthesis Method for the Efficient Production of Synthetic Protein Libraries for Directed Evolution

    4. No Access

      Protocol

      Pages 79-91

      BASIC: A Simple and Accurate Modular DNA Assembly Method

    5. No Access

      Protocol

      Pages 93-103

      Enzymatic Synthesis of Single-Stranded Clonal Pure Oligonucleotides

    6. No Access

      Protocol

      Pages 105-110

      Rapid Assembly of DNA via Ligase Cycling Reaction (LCR)

    7. No Access

      Protocol

      Pages 111-128

      PaperClip: A Simple Method for Flexible Multi-Part DNA Assembly

    8. No Access

      Protocol

      Pages 129-138

      The Polymerase Step Reaction (PSR) Method for Gene and Library Synthesis

    9. No Access

      Protocol

      Pages 139-155

      Clonetegration Using OSIP Plasmids: One-Step DNA Assembly and Site-Specific Genomic Integration in Bacteria

    10. No Access

      Protocol

      Pages 157-168

      Generation of DNA Constructs Using the Golden GATEway Cloning Method

    11. No Access

      Protocol

      Pages 169-185

      Gene Deletion by Synthesis in Yeast

    12. No Access

      Protocol

      Pages 187-192

      Efficient Assembly of DNA Using Yeast Homologous Recombination (YHR)

    13. No Access

      Protocol

      Pages 193-203

      Simultaneous Removal of Multiple DNA Segments by Polymerase Chain Reactions

    14. No Access

      Protocol

      Pages 205-214

      Rapid Construction of Recombinant Plasmids by QuickStep-Cloning

  4. Reducing Error in Synthetic DNA

    1. Front Matter

      Pages 215-215

    2. No Access

      Protocol

      Pages 217-235

      Immobilized MutS-Mediated Error Removal of Microchip-Synthesized DNA

    3. No Access

      Protocol

      Pages 237-246

      Selection of Error-Less Synthetic Genes in Yeast

  5. Back Matter

    Pages 247-248