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Functional Measurement of Respiratory Muscle Motor Behaviors Using Transdiaphragmatic Pressure

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Skeletal Muscle Regeneration in the Mouse

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1460))

Abstract

The diaphragm muscle must be able to generate sufficient forces to accomplish a range of ventilatory and non-ventilatory behaviors throughout life. Measurements of transdiaphragmatic pressure (Pdi) can be conducted during eupnea, hypoxia (10 % O2)–hypercapnia (5 % CO2), chemical airway stimulation (i.e., sneezing), spontaneously occurring deep breaths (i.e., sighs), sustained airway or tracheal occlusion, and maximal efforts elicited via bilateral phrenic nerve stimulation, representing the full range of motor behaviors available by the diaphragm muscle. We provide detailed methods on the in vivo measurements of Pdi in mice.

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Acknowledgments

Any procedures conducted on animals in the development of these methods were conducted following institutional protocols and animal care guidelines, in compliance with National Institute of Health Guidelines.

This work was supported by grants from National Institute of Health R01-AG-044615 and R01-HL-096750 (CBM and GCS), T32-HL105355 (SMG), and the Mayo Clinic.

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Authors and Affiliations

  1. Department of Physiology and Biomedical Engineering, Mayo Clinic, 200 First Street SW, SMH Jo 4-184, Rochester, MN, 55905, USA

    Sarah M. Greising, Carlos B. Mantilla & Gary C. Sieck

  2. Department of Anesthesiology, Mayo Clinic, 200 First Street SW, SMH Jo 4-184, Rochester, MN, 55905, USA

    Carlos B. Mantilla & Gary C. Sieck

Authors
  1. Sarah M. Greising
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  2. Carlos B. Mantilla
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  3. Gary C. Sieck
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Corresponding author

Correspondence to Gary C. Sieck .

Editor information

Editors and Affiliations

  1. Lillehei Heart Institute, University of Minnesota, Minneapolis, Minnesota, USA

    Michael Kyba

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Greising, S.M., Mantilla, C.B., Sieck, G.C. (2016). Functional Measurement of Respiratory Muscle Motor Behaviors Using Transdiaphragmatic Pressure. In: Kyba, M. (eds) Skeletal Muscle Regeneration in the Mouse. Methods in Molecular Biology, vol 1460. Springer, New York, NY. https://doi.org/10.1007/978-1-4939-3810-0_21

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  • DOI: https://doi.org/10.1007/978-1-4939-3810-0_21

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  • Publisher Name: Springer, New York, NY

  • Print ISBN: 978-1-4939-3808-7

  • Online ISBN: 978-1-4939-3810-0

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