Skeletal Muscle Regeneration in the Mouse

Methods and Protocols

  • Michael Kyba

Part of the Methods in Molecular Biology book series (MIMB, volume 1460)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Injury Models

    1. Front Matter
      Pages 1-1
    2. Beth E. Pollot, Benjamin T. Corona
      Pages 19-31
    3. Gengyun Le, Dawn A. Lowe, Michael Kyba
      Pages 33-41
    4. Tyler J. Kirby, John J. McCarthy, Charlotte A. Peterson, Christopher S. Fry
      Pages 43-52
    5. Glynnis A. Garry, Marie Lue Antony, Daniel J. Garry
      Pages 61-71
  3. Evaluating Progenitor Cells

    1. Front Matter
      Pages 83-83
    2. Huascar P. Ortuste Quiroga, Katsumasa Goto, Peter S. Zammit
      Pages 85-100
    3. Mayank Verma, Bhavani SR Murkonda, Yoko Asakura, Atsushi Asakura
      Pages 129-140
    4. Thomas O. Vogler, Katherine E. Gadek, Adam B. Cadwallader, Tiffany L. Elston, Bradley B. Olwin
      Pages 141-162
    5. Alessandro Magli, Tania Incitti, Rita C. R. Perlingeiro
      Pages 191-208
    6. Laura García-Prat, Marta Martínez-Vicente, Pura Muñoz-Cánoves
      Pages 223-240

About this book

Introduction

This volume focuses on the cell biology and physiology of skeletal muscle regeneration. This Book is a collection of classic and cutting edge protocols optimized for mice, but in most cases adaptable to rat or other mammalian models, that will allow an investigator to develop and implement a research study on skeletal muscle regeneration. Chapters address the three major areas of study: provoking regeneration by inducing damage to muscle, analyzing the progenitor cells of skeletal muscle, and quantifying overall muscle function. Subjects discussed include: inducing skeletal muscle injury by eccentric contraction; volumetric muscle loss; single myofiber isolation and culture; satellite cell transplantation; muscle clearing for whole mount immunostaining; luciferase tracking of muscle stem cells; mitochondrial and mitophagy flux analysis; in vivo assessment of muscle contractility; force measurements on single isolated myofibers; and analysis of aerobic respiration in intact skeletal muscle tissue by microplate respirometry. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to each respective topic, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting edge and practical, Skeletal Muscle Regeneration in the Mouse: Methods and Protocols is an essential laboratory reference for research in skeletal muscle growth, damage, repair, degeneration, and regenerative therapy in the mouse model system.

Keywords

degeneration tissue repair skeletal muscle progenitor cells skeletal muscle growth regenerative therapy muscle Injury immunofluorescence staining satellite cells

Editors and affiliations

  • Michael Kyba
    • 1
  1. 1.Lillehei Heart InstituteUniversity of MinnesotaMinneapolisUSA

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4939-3810-0
  • Copyright Information Springer Science+Business Media New York 2016
  • Publisher Name Springer, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-3808-7
  • Online ISBN 978-1-4939-3810-0
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book