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Construction of Tissue-Engineered Nerve Conduits Seeded with Neurons Derived from Hair-Follicle Neural Crest Stem Cells

  • Fang LiuEmail author
  • Haiyan Lin
  • Chuansen ZhangEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1453)

Abstract

Tissue-engineered nerve conduits are widely used for the study of peripheral nerve injury repair. With regard to repairing long nerve defects, stem-cell-derived neurons are recommended as seed cells. As hair-follicle neural crest stem cells (hfNCSCs) are easily to be harvested from patients and have the potential to differentiate into neuronal cells, hfNCSCs-derived neurons are an ideal candidate choice. Acellular nerve grafts, a type of biological material scaffold, with intact collagen structure, with biocompatibility and less toxicity are obtained through removing live cells with 1 % lysolecithin, are also an ideal choice. In the present report, we describe a tissue-engineered nerve conduit seeded with rat hfNCSCs-derived neurons into the beagle acellular sciatic nerve scaffold. Our goal is to provide a novel engineered therapeutic for repairing peripheral nerve injury with long distance defects.

Key words

Hair follicle Neural crest stem cells Neuron Acellular nerve scaffold Tissue-engineered nerve conduit Nerve injury repair 

Notes

Acknowledgement

This work was supported by the National Natural Science Foundation of China (no. 81571211) and the Natural Science Foundation of Shanghai, China (no. 14ZR1449300).

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  1. 1.Department of AnatomyInstitute of Biomedical Engineering, Second Military Medical UniversityShanghaiPeople’s Republic of China
  2. 2.AntiCancer, Inc.San DiegoUSA

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