Abstract
Tissue-engineered nerve conduits are widely used for the study of peripheral nerve injury repair. With regard to repairing long nerve defects, stem-cell-derived neurons are recommended as seed cells. As hair-follicle neural crest stem cells (hfNCSCs) are easily to be harvested from patients and have the potential to differentiate into neuronal cells, hfNCSCs-derived neurons are an ideal candidate choice. Acellular nerve grafts, a type of biological material scaffold, with intact collagen structure, with biocompatibility and less toxicity are obtained through removing live cells with 1 % lysolecithin, are also an ideal choice. In the present report, we describe a tissue-engineered nerve conduit seeded with rat hfNCSCs-derived neurons into the beagle acellular sciatic nerve scaffold. Our goal is to provide a novel engineered therapeutic for repairing peripheral nerve injury with long distance defects.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Xu L, Zhou S, Feng GY et al (2012) Neural stem cells enhance nerve regeneration after sciatic nerve injury in rats. Mol Neurobiol 46(2):265–274
Uemura T, Takamatsu K, Ikeda M et al (2012) Transplantation of induced pluripotent stem cell-derived neurospheres for peripheral nerve repair. Biochem Biophys Res Commun 419(1):130–135
Liu BS, Yang YC, Shen CC (2012) Regenerative effect of adipose tissue-derived stem cells transplantation using nerve conduit therapy on sciatic nerve injury in rats. J Tissue Eng Regen Med 8:337–350. doi:10.1002/term.1523 [Epub ahead of print]
Ding F, Wu J, Yang Y et al (2010) Use of tissue-engineered nerve grafts consisting of a chitosan/poly(lactic-co-glycolic acid)-based scaffold included with bone marrow mesenchymal cells for bridging 50-mm dog sciatic nerve gaps. Tissue Eng Part A 16(12):3779–3790
Amoh Y, Li L, Campillo R et al (2005) Implanted hair follicle stem cells from Schwann cells that support repair of severed peripheral nerves. Proc Natl Acad Sci U S A 102(49):17734–17738
Thomas CK, Sesodia S, Erb DE et al (2003) Properties of medial gastrocnemius motor units and muscle fibers reinnervated by embryonic ventral spinal cord cells. Exp Neurol 180(1):25–31
MacDonald SC, Fleetwood IG, Hochman S et al (2003) Functional motor neurons differentiating from mouse multipotent spinal cord precursor cells in culture and after transplantation into transected sciatic nerve. J Neurosurg 98:1094–1103
Yohn DC, Miles GB, Rafuse VF et al (2008) Transplanted mouse embryonic stem-cell-derived motoneurons form functional motor units and reduce muscle atrophy. J Neurosci 28(47):12409–12418
Gu SH, Xu WD, Xu L et al (2010) Regenerated host axons form synapses with neurons derived from neural stem cells transplanted into peripheral nerves. J Int Med Res 38:1721–1729
Lin H, Liu F, Zhang C et al (2009) Pluripotent hair follicle neural crest stem cell-derived neurons and Schwann cells functionally repair sciatic nerves in rats. Mol Neurobiol 40(3):216–223
Li L, Mignone J, Yang M, Matic M, Penman S, Enikolopov G, Hoffman RM (2003) Nestin expression in hair follicle sheath progenitor cells. Proc Natl Acad Sci USA 100, 9958–9961
Amoh Y, Li L, Katsuoka K et al (2005) Multipotent nestin-positive, keratin-negative hair follicle bulge stem cells can form neurons. Proc Natl Acad Sci U S A 102(15):5530–5534
Lin H, Liu F, Zhang C et al (2011) Characterization of nerve conduits seeded with neurons and Schwann cells derived from hair follicle neural crest stem cells. Tissue Eng Part A 17(13–14):1691–1698
Hudson TW, Evans GR, Schmidt CE (2000) Engineering strategies for peripheral nerve repair. Orthop Clin North Am 31:485
Schmidt CE, Leach JB (2003) Neural tissue engineering: strategies for repair and regeneration. Annu Rev Biomed Eng 5:293
Taras JS, Nanavati V, Steelman P (2005) Nerve conduits. J Hand Ther 18:191
Dumont CE, Hentz VR (1997) Enhancement of axon growth by detergent-extracted nerve grafts. Transplantation 63:1210
Acknowledgement
This work was supported by the National Natural Science Foundation of China (no. 81571211) and the Natural Science Foundation of Shanghai, China (no. 14ZR1449300).
Author information
Authors and Affiliations
Corresponding authors
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2016 Springer Science+Business Media New York
About this protocol
Cite this protocol
Liu, F., Lin, H., Zhang, C. (2016). Construction of Tissue-Engineered Nerve Conduits Seeded with Neurons Derived from Hair-Follicle Neural Crest Stem Cells. In: Hoffman, R. (eds) Multipotent Stem Cells of the Hair Follicle. Methods in Molecular Biology, vol 1453. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3786-8_5
Download citation
DOI: https://doi.org/10.1007/978-1-4939-3786-8_5
Published:
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-3784-4
Online ISBN: 978-1-4939-3786-8
eBook Packages: Springer Protocols