Abstract
The physiological role of histone deacetylase 11 (HDAC11), the newest member of the HDAC family, remained largely unknown until the discovery of its regulatory function in immune cells. Among them, the regulation of cytokine production by antigen-presenting cells and the modulation of the suppressive ability of myeloid-derived suppressor cells (MDSCs) (Sahakian et al. Mol Immunol 63: 579–585, 2015; Wang et al. J Immunol 186: 3986–3996, 2011; Villagra et al. Nat Immunol 10: 92–100, 2009). Our earlier data has demonstrated that HDAC11, by interacting at the chromatin level with the IL-10 promoter, downregulates il-10 transcription in both murine and human APCs in vitro and ex vivo models (Villagra et al. Nat Immunol 10: 92–100, 2009). However the role of HDAC11 in other cell types still remains unknown. Here we present several methods that can potentially be used to identify the functional role of HDAC11, assigning special attention to the evaluation of immunological parameters.
The original version of this chapter was revised. The erratum to this chapter is available at: DOI 10.1007/978-1-4939-3667-0_22
An erratum to this chapter can be found at http://dx.doi.org/10.1007/978-1-4939-3667-0_22
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References
Villagra A, Sotomayor EM, Seto E (2010) Histone deacetylases and the immunological network: implications in cancer and inflammation. Oncogene 29(2):157–173
Foster SL, Hargreaves DC, Medzhitov R (2007) Gene-specific control of inflammation by TLR-induced chromatin modifications. Nature 447(7147):972–978
Woan KV, Sahakian E, Sotomayor EM, Seto E, Villagra A (2012) Modulation of antigen-presenting cells by HDAC inhibitors: implications in autoimmunity and cancer. Immunol Cell Biol 90(1):55–65
Miremadi A, Oestergaard MZ, Pharoah PDP, Caldas C (2007) Cancer genetics of epigenetic genes. Hum Mol Genet 16(R1):R28–R49
Schreiber RD, Old LJ, Smyth MJ (2011) Cancer immunoediting: integrating immunity’s roles in cancer suppression and promotion. Science 331(6024):1565–1570
Gao L, Cueto MA, Asselbergs F, Atadja P (2002) Cloning and functional characterization of HDAC11, a novel member of the human histone deacetylase family. J Biol Chem 277(28):25748–25755
Sahakian E, Powers JJ, Chen J et al (2014) Histone deacetylase 11: a novel epigenetic regulator of myeloid derived suppressor cell expansion and function. Mol Immunol 63(2):579–585
Cheng F, Lienlaf M, Perez-Villarroel P et al (2014) Divergent roles of histone deacetylase 6 (HDAC6) and histone deacetylase 11 (HDAC11) on the transcriptional regulation of IL10 in antigen presenting cells. Mol Immunol 60(1):44–53
Gong S, Zheng C, Doughty ML et al (2003) A gene expression atlas of the central nervous system based on bacterial artificial chromosomes. Nature 425(6961):917–925
Heintz N (2001) BAC to the future: the use of bac transgenic mice for neuroscience research. Nat Rev Neurosci 2(12):861–870
Sahakian E, Powers JJ, Chen J et al (2015) Histone deacetylase 11: a novel epigenetic regulator of myeloid derived suppressor cell expansion and function. Mol Immunol 63(2):579–585
Wang H, Cheng F, Woan K et al (2011) Histone deacetylase inhibitor LAQ824 augments inflammatory responses in macrophages through transcriptional regulation of IL-10. J Immunol 186(7):3986–3996
Villagra A, Cheng F, Wang HW et al (2009) The histone deacetylase HDAC11 regulates the expression of interleukin 10 and immune tolerance. Nat Immunol 10(1):92–100
Cheng F, Lienlaf M, Wang HW et al (2014) A novel role for histone deacetylase 6 in the regulation of the tolerogenic STAT3/IL-10 pathway in APCs. J Immunol 193(6):2850–2862
Cheng F, Wang H, Horna P et al (2012) Stat3 inhibition augments the immunogenicity of B-cell lymphoma cells, leading to effective antitumor immunity. Cancer Res 72(17):4440–4448
Eva Sahakian JC, John J. Powers, Xianghong Chen, Kamira Maharaj, Susan L. Deng, Maritza Lienlaf, Hong Wei Wang, Andressa L. Sodré, Allison Distler, Limin Xing, Patricio Perez-Villarroel, Sheng Wei, Alejandro Villagra, Ed Seto, Eduardo M. Sotomayor, Pedro Horna and Javier Pinilla-Ibarz (2015) Essential regulatory role for histone deacetylase 11 (HDAC11) In neutrophil function. J Leukoc Biol. Under 2nd revision
Lee TI, Johnstone SE, Young RA (2006) Chromatin immunoprecipitation and microarray-based analysis of protein location. Nat Protoc 1(2):729–748
Nelson JD, Denisenko O, Bomsztyk K (2006) Protocol for the fast chromatin immunoprecipitation (ChIP) method. Nat Protoc 1(1):179–185
Acknowledgments
The authors gratefully acknowledge the flow cytometry core facilities at H. Lee Moffitt Cancer Center and their extended technical support for our project.
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Chen, J. et al. (2016). Functional Analysis of Histone Deacetylase 11 (HDAC11). In: Sarkar, S. (eds) Histone Deacetylases. Methods in Molecular Biology, vol 1436. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3667-0_11
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DOI: https://doi.org/10.1007/978-1-4939-3667-0_11
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