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Testing the Paracrine Properties of Human Mesenchymal Stem Cells Using Conditioned Medium

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Mesenchymal Stem Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1416))

Abstract

Mesenchymal stem cells (MSC) produce and secrete a great variety of cytokines and chemokines that play beneficial paracrine actions when MSC are used for tissue repair. The conditioned medium (CM) derived from MSC can be used both in vitro and in vivo to test specific paracrine effects or to screen putative paracrine/autocrine mediators by proteomics.

In this chapter, we describe a straightforward method to prepare MSC-derived CM. Furthermore, we summarize some in vitro assays useful for testing the cytoprotective, angiogenic, and regenerative activity of CM. These assays are very helpful when studying the role of MSC in cardiac repair and regeneration.

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References

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Acknowledgments

This work was supported by the Ministero Italiano della Sanità [Grant number GR-2008-1142871 and GR-2010-2320533]; the Fondazione Cariplo [Grant number 2007-5984], and the Ministero Italiano degli Affari Esteri [Grant number ZA11GR2].

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Authors and Affiliations

  1. Department of Molecular Medicine, Unit of Cardiology, University of Pavia, Pavia, Italy

    Patrizia Danieli Ph.D., Giuseppe Malpasso Ph.D., Maria Chiara Ciuffreda Ph.D. & Massimiliano Gnecchi M.D., Ph.D., F.E.S.C.

  2. Department of Cardiothoracic and Vascular Sciences – Coronary Care Unit and Laboratory of Clinical and Experimental Cardiology, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy

    Patrizia Danieli Ph.D., Giuseppe Malpasso Ph.D., Maria Chiara Ciuffreda Ph.D. & Massimiliano Gnecchi M.D., Ph.D., F.E.S.C.

  3. Laboratory of Experimental Cardiology for Cell and Molecular Therapy, Department of Cardiology, Fondazione IRCCS Policlinico San Matteo, Viale Golgi 19, Pavia, 27100, Italy

    Patrizia Danieli Ph.D., Giuseppe Malpasso Ph.D., Maria Chiara Ciuffreda Ph.D. & Massimiliano Gnecchi M.D., Ph.D., F.E.S.C.

  4. Department of Medicine, University of Cape Town, Cape Town, South Africa

    Massimiliano Gnecchi M.D., Ph.D., F.E.S.C.

Authors
  1. Patrizia Danieli Ph.D.
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  2. Giuseppe Malpasso Ph.D.
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  3. Maria Chiara Ciuffreda Ph.D.
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  4. Massimiliano Gnecchi M.D., Ph.D., F.E.S.C.
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Corresponding author

Correspondence to Massimiliano Gnecchi M.D., Ph.D., F.E.S.C. .

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Editors and Affiliations

  1. Dept of Molecular Med,Unit of Cardiology, University of Pavia, PAVIA, Italy

    Massimiliano Gnecchi

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Danieli, P., Malpasso, G., Ciuffreda, M.C., Gnecchi, M. (2016). Testing the Paracrine Properties of Human Mesenchymal Stem Cells Using Conditioned Medium. In: Gnecchi, M. (eds) Mesenchymal Stem Cells. Methods in Molecular Biology, vol 1416. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3584-0_26

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  • DOI: https://doi.org/10.1007/978-1-4939-3584-0_26

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3582-6

  • Online ISBN: 978-1-4939-3584-0

  • eBook Packages: Springer Protocols

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